Branching morphogenesis of immortalized human bronchial epithelial cells in three-dimensional culture

Aadil Kaisani, Oliver Delgado, Gail Fasciani, Sang Bum Kim, Woodring E. Wright, John D. Minna, Jerry W. Shay

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

While mouse models have contributed in our understanding of lung development, repair and regeneration, inherent differences between the murine and human airways requires the development of new models using human airway epithelial cells. In this study, we describe a three-dimensional model system using human bronchial epithelial cells (HBECs) cultured on reconstituted basement membrane. HBECs form complex budding and branching structures on reconstituted basement membrane when co-cultured with human lung fetal fibroblasts. These structures are reminiscent of the branching epithelia during lung development. The HBECs also retain markers indicative of epithelial cell types from both the central and distal airways suggesting their multipotent potential. In addition, we illustrate how the model can be utilized to understand respiratory diseases such as lung cancer. The 3D novel cell culture system recapitulates stromal-epithelial interactions in vitro that can be utilized to understand important aspects of lung development and diseases.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalDifferentiation
Volume87
Issue number3-4
DOIs
StatePublished - 2014

Fingerprint

Morphogenesis
Epithelial Cells
Basement Membrane
Lung
Lung Diseases
Regeneration
Lung Neoplasms
Epithelium
Cell Culture Techniques
Fibroblasts

Keywords

  • Branching
  • Bronchial epithelial cells
  • Differentiation
  • Distal airways
  • Fibroblasts

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology
  • Cancer Research
  • Medicine(all)

Cite this

Branching morphogenesis of immortalized human bronchial epithelial cells in three-dimensional culture. / Kaisani, Aadil; Delgado, Oliver; Fasciani, Gail; Kim, Sang Bum; Wright, Woodring E.; Minna, John D.; Shay, Jerry W.

In: Differentiation, Vol. 87, No. 3-4, 2014, p. 119-126.

Research output: Contribution to journalArticle

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