Breaking the spatial resolution barrier via iterative sound-light interaction in deep tissue microscopy

Ke Si, Reto Fiolka, Meng Cui

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Optical microscopy has so far been restricted to superficial layers, leaving many important biological questions unanswered. Random scattering causes the ballistic focus, which is conventionally used for image formation, to decay exponentially with depth. Optical imaging beyond the ballistic regime has been demonstrated by hybrid techniques that combine light with the deeper penetration capability of sound waves. Deep inside highly scattering media, the sound focus dimensions restrict the imaging resolutions. Here we show that by iteratively focusing light into an ultrasound focus via phase conjugation, we can fundamentally overcome this resolution barrier in deep tissues and at the same time increase the focus to background ratio. We demonstrate fluorescence microscopy beyond the ballistic regime of light with a threefold improved resolution and a fivefold increase in contrast. This development opens up practical high resolution fluorescence imaging in deep tissues.

Original languageEnglish (US)
Article number748
JournalScientific Reports
Volume2
DOIs
StatePublished - 2012

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Microscopy
Optical Imaging
Light
Fluorescence Microscopy

ASJC Scopus subject areas

  • General

Cite this

Breaking the spatial resolution barrier via iterative sound-light interaction in deep tissue microscopy. / Si, Ke; Fiolka, Reto; Cui, Meng.

In: Scientific Reports, Vol. 2, 748, 2012.

Research output: Contribution to journalArticle

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