BREs mediate both repression and activation of oskar mRNA translation and act in trans.

Brad Reveal, Nan Yan, Mark J. Snee, Chin I. Pai, Youme Gim, Paul M. Macdonald

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Asymmetric positioning of proteins within cells is crucial for cell polarization and function. Deployment of Oskar protein at the posterior pole of the Drosophila oocyte relies on localization of the oskar mRNA, repression of its translation prior to localization, and finally activation of translation. Translational repression is mediated by BREs, regulatory elements positioned in two clusters near both ends of the oskar mRNA 3' UTR. Here we show that some BREs are bifunctional: both clusters of BREs contribute to translational repression, and the 3' cluster has an additional role in release from BRE-dependent repression. Remarkably, both BRE functions can be provided in trans by an oskar mRNA with wild-type BREs that is itself unable to encode Oskar protein. Regulation in trans is likely enabled by assembly of oskar transcripts in cytoplasmic RNPs. Concentration of transcripts in such RNPs is common, and trans regulation of mRNAs may therefore be widespread.

Original languageEnglish (US)
Pages (from-to)496-502
Number of pages7
JournalDevelopmental Cell
Volume18
Issue number3
StatePublished - Mar 16 2010

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Protein Biosynthesis
Chemical activation
Messenger RNA
Proteins
3' Untranslated Regions
Drosophila
Oocytes
Poles
Polarization

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Reveal, B., Yan, N., Snee, M. J., Pai, C. I., Gim, Y., & Macdonald, P. M. (2010). BREs mediate both repression and activation of oskar mRNA translation and act in trans. Developmental Cell, 18(3), 496-502.

BREs mediate both repression and activation of oskar mRNA translation and act in trans. / Reveal, Brad; Yan, Nan; Snee, Mark J.; Pai, Chin I.; Gim, Youme; Macdonald, Paul M.

In: Developmental Cell, Vol. 18, No. 3, 16.03.2010, p. 496-502.

Research output: Contribution to journalArticle

Reveal, B, Yan, N, Snee, MJ, Pai, CI, Gim, Y & Macdonald, PM 2010, 'BREs mediate both repression and activation of oskar mRNA translation and act in trans.', Developmental Cell, vol. 18, no. 3, pp. 496-502.
Reveal B, Yan N, Snee MJ, Pai CI, Gim Y, Macdonald PM. BREs mediate both repression and activation of oskar mRNA translation and act in trans. Developmental Cell. 2010 Mar 16;18(3):496-502.
Reveal, Brad ; Yan, Nan ; Snee, Mark J. ; Pai, Chin I. ; Gim, Youme ; Macdonald, Paul M. / BREs mediate both repression and activation of oskar mRNA translation and act in trans. In: Developmental Cell. 2010 ; Vol. 18, No. 3. pp. 496-502.
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