BRI3 associates with SCG10 and attenuates NGF-induced neurite outgrowth in PC12 cells

Yanhua Gong, Jing Wu, Hua Qiang, Ben Liu, Zhikai Chi, Tao Chen, Bin Yin, Xiaozhong Peng, Jiangang Yuan

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

In a yeast two-hybrid screen, we identified the microtubule-destabilizing protein SCG10 as a potential effector protein of BRI3. The association was verified using GST pull-down, Co-IP, and their perinuclear co-localization. The analysis of in vitro microtubule polymerization/depolymerization showed that the binding of BRI3 to SCG10 effectively blocked the ability of SCG10 to induce microtubule disassembly, as determined by turbidimetric assays. In intact PC12 cells, BRI3 exhibited the ability to stabilize the microtubule network and attenuate the microtubule-destabilizing activity of SCG10. Furthermore, co-expression of BRI3 with SCG10 attenuated SCG10-mediated PC12 cell neurite outgrowth induced by NGF. These results identify a novel connection between a neuron-specific BRI protein and the cytoskeletal network, suggesting possible roles of BRI3 in the process of neuronal differentiation.

Original languageEnglish (US)
Pages (from-to)287-293
Number of pages7
JournalJournal of Biochemistry and Molecular Biology
Volume41
Issue number4
StatePublished - Apr 1 2008
Externally publishedYes

Keywords

  • Cytoskeletal network
  • Microtubule
  • Neurite outgrowth
  • Neuronal differentiation
  • PC12

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Gong, Y., Wu, J., Qiang, H., Liu, B., Chi, Z., Chen, T., Yin, B., Peng, X., & Yuan, J. (2008). BRI3 associates with SCG10 and attenuates NGF-induced neurite outgrowth in PC12 cells. Journal of Biochemistry and Molecular Biology, 41(4), 287-293.