Brn3a and Klf7 cooperate to control TrkA expression in sensory neurons

Lei Lei, Jing Zhou, Lu Lin, Luis F. Parada

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


The zinc finger protein Klf7 and POU homeodomain protein Brn3a are each required for efficient transcription of TrkA in primary sensory neurons. In this study, we examined whether these transcription factors act in concert to regulate TrkA expression. In vitro, Brn3a and Klf7 can synergistically activate the TrkA enhancer. In vivo, precursor cells that are destined to become TrkA(+) neurons are born. However, both Brn3a and Klf7 are dispensable for the initiation of TrkA expression. At E12.5, while TrkA expression is unaffected in Brn3a-/- trigeminal ganglia and only slightly decreased in Klf7-/- trigeminal ganglia, it is severely reduced in the double mutant Brn3a-/-;Klf7-/- trigeminal ganglia. At birth, all Trk(+) neurons are lost in Brn3a-/-;Klf7-/- trigeminal ganglia. We further demonstrate that the TrkA enhancer is inactive in Brn3a-/-;Klf7-/- trigeminal ganglia. Thus, cooperation between these two transcription factors is required for endogenous TrkA gene expression and the survival of nociceptive sensory neurons.

Original languageEnglish (US)
Pages (from-to)758-769
Number of pages12
JournalDevelopmental Biology
Issue number2
StatePublished - Dec 15 2006


  • Apoptosis
  • Brn3a
  • Klf7
  • Neurotrophin
  • Sensory neuron
  • Transcription
  • TrkA
  • TrkB
  • TrkC

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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