Brn3a and Klf7 cooperate to control TrkA expression in sensory neurons

Lei Lei, Jing Zhou, Lu Lin, Luis F. Parada

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The zinc finger protein Klf7 and POU homeodomain protein Brn3a are each required for efficient transcription of TrkA in primary sensory neurons. In this study, we examined whether these transcription factors act in concert to regulate TrkA expression. In vitro, Brn3a and Klf7 can synergistically activate the TrkA enhancer. In vivo, precursor cells that are destined to become TrkA(+) neurons are born. However, both Brn3a and Klf7 are dispensable for the initiation of TrkA expression. At E12.5, while TrkA expression is unaffected in Brn3a-/- trigeminal ganglia and only slightly decreased in Klf7-/- trigeminal ganglia, it is severely reduced in the double mutant Brn3a-/-;Klf7-/- trigeminal ganglia. At birth, all Trk(+) neurons are lost in Brn3a-/-;Klf7-/- trigeminal ganglia. We further demonstrate that the TrkA enhancer is inactive in Brn3a-/-;Klf7-/- trigeminal ganglia. Thus, cooperation between these two transcription factors is required for endogenous TrkA gene expression and the survival of nociceptive sensory neurons.

Original languageEnglish (US)
Pages (from-to)758-769
Number of pages12
JournalDevelopmental Biology
Volume300
Issue number2
DOIs
StatePublished - Dec 15 2006

Fingerprint

Trigeminal Ganglion
Sensory Receptor Cells
Transcription Factors
POU Domain Factors
Homeodomain Proteins
Neurons
Nociceptors
Zinc Fingers
Parturition
Gene Expression
Proteins

Keywords

  • Apoptosis
  • Brn3a
  • Klf7
  • Neurotrophin
  • Sensory neuron
  • Transcription
  • TrkA
  • TrkB
  • TrkC

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Brn3a and Klf7 cooperate to control TrkA expression in sensory neurons. / Lei, Lei; Zhou, Jing; Lin, Lu; Parada, Luis F.

In: Developmental Biology, Vol. 300, No. 2, 15.12.2006, p. 758-769.

Research output: Contribution to journalArticle

Lei, Lei ; Zhou, Jing ; Lin, Lu ; Parada, Luis F. / Brn3a and Klf7 cooperate to control TrkA expression in sensory neurons. In: Developmental Biology. 2006 ; Vol. 300, No. 2. pp. 758-769.
@article{e93c6a0548a04b8382712bd26f06cba6,
title = "Brn3a and Klf7 cooperate to control TrkA expression in sensory neurons",
abstract = "The zinc finger protein Klf7 and POU homeodomain protein Brn3a are each required for efficient transcription of TrkA in primary sensory neurons. In this study, we examined whether these transcription factors act in concert to regulate TrkA expression. In vitro, Brn3a and Klf7 can synergistically activate the TrkA enhancer. In vivo, precursor cells that are destined to become TrkA(+) neurons are born. However, both Brn3a and Klf7 are dispensable for the initiation of TrkA expression. At E12.5, while TrkA expression is unaffected in Brn3a-/- trigeminal ganglia and only slightly decreased in Klf7-/- trigeminal ganglia, it is severely reduced in the double mutant Brn3a-/-;Klf7-/- trigeminal ganglia. At birth, all Trk(+) neurons are lost in Brn3a-/-;Klf7-/- trigeminal ganglia. We further demonstrate that the TrkA enhancer is inactive in Brn3a-/-;Klf7-/- trigeminal ganglia. Thus, cooperation between these two transcription factors is required for endogenous TrkA gene expression and the survival of nociceptive sensory neurons.",
keywords = "Apoptosis, Brn3a, Klf7, Neurotrophin, Sensory neuron, Transcription, TrkA, TrkB, TrkC",
author = "Lei Lei and Jing Zhou and Lu Lin and Parada, {Luis F.}",
year = "2006",
month = "12",
day = "15",
doi = "10.1016/j.ydbio.2006.08.062",
language = "English (US)",
volume = "300",
pages = "758--769",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Brn3a and Klf7 cooperate to control TrkA expression in sensory neurons

AU - Lei, Lei

AU - Zhou, Jing

AU - Lin, Lu

AU - Parada, Luis F.

PY - 2006/12/15

Y1 - 2006/12/15

N2 - The zinc finger protein Klf7 and POU homeodomain protein Brn3a are each required for efficient transcription of TrkA in primary sensory neurons. In this study, we examined whether these transcription factors act in concert to regulate TrkA expression. In vitro, Brn3a and Klf7 can synergistically activate the TrkA enhancer. In vivo, precursor cells that are destined to become TrkA(+) neurons are born. However, both Brn3a and Klf7 are dispensable for the initiation of TrkA expression. At E12.5, while TrkA expression is unaffected in Brn3a-/- trigeminal ganglia and only slightly decreased in Klf7-/- trigeminal ganglia, it is severely reduced in the double mutant Brn3a-/-;Klf7-/- trigeminal ganglia. At birth, all Trk(+) neurons are lost in Brn3a-/-;Klf7-/- trigeminal ganglia. We further demonstrate that the TrkA enhancer is inactive in Brn3a-/-;Klf7-/- trigeminal ganglia. Thus, cooperation between these two transcription factors is required for endogenous TrkA gene expression and the survival of nociceptive sensory neurons.

AB - The zinc finger protein Klf7 and POU homeodomain protein Brn3a are each required for efficient transcription of TrkA in primary sensory neurons. In this study, we examined whether these transcription factors act in concert to regulate TrkA expression. In vitro, Brn3a and Klf7 can synergistically activate the TrkA enhancer. In vivo, precursor cells that are destined to become TrkA(+) neurons are born. However, both Brn3a and Klf7 are dispensable for the initiation of TrkA expression. At E12.5, while TrkA expression is unaffected in Brn3a-/- trigeminal ganglia and only slightly decreased in Klf7-/- trigeminal ganglia, it is severely reduced in the double mutant Brn3a-/-;Klf7-/- trigeminal ganglia. At birth, all Trk(+) neurons are lost in Brn3a-/-;Klf7-/- trigeminal ganglia. We further demonstrate that the TrkA enhancer is inactive in Brn3a-/-;Klf7-/- trigeminal ganglia. Thus, cooperation between these two transcription factors is required for endogenous TrkA gene expression and the survival of nociceptive sensory neurons.

KW - Apoptosis

KW - Brn3a

KW - Klf7

KW - Neurotrophin

KW - Sensory neuron

KW - Transcription

KW - TrkA

KW - TrkB

KW - TrkC

UR - http://www.scopus.com/inward/record.url?scp=33845393338&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845393338&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2006.08.062

DO - 10.1016/j.ydbio.2006.08.062

M3 - Article

C2 - 17011544

AN - SCOPUS:33845393338

VL - 300

SP - 758

EP - 769

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -