Abstract
Accurate partition of duplicated genetic material to the daughter cells during mitosis relies on the maintenance of the physical linkage (cohesion) between sister chromatids until their bipolar attachment to the mitotic spindle. In response to a single straying chromatid within a cell, a surveillance mechanism called the spindle checkpoint blocks the ubiquitin ligase activity of the anaphase-promoting complex or cyclosome (APC/C), stabilizes securin (an APC/C substrate and an inhibitor of separase), and delays the activation of separase. This in turn prevents cleavage of cohesin by separase, preserves sister chromatid cohesion, and delays the onset of anaphase. The protein kinase, Bub1, is a key component of the spindle checkpoint. Bub1 has an upstream function in regulating the kinetochore localization of Mad2 and other downstream checkpoint components. In addition, recent biochemical studies have shown that Bub1 directly phosphorylates the APC/C activator, Cdc20, and inhibits APC/C. Finally, Bub1 has a noncheckpoint function at the kinetochores and preserves centromeric cohesion through the MEI-S332/shugoshin family of proteins. Therefore, Bub1 performs multiple tasks in mitosis that ensure the proper inheritance of chromosomes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 262-265 |
| Number of pages | 4 |
| Journal | Cell Cycle |
| Volume | 4 |
| Issue number | 2 |
| State | Published - Feb 2005 |
Fingerprint
Keywords
- Anaphase-promoting complex
- Bub1
- Cdc20
- Cohesin
- Cyclosome
- MEI-S332
- Mitosis
- Shugoshin
- Sister chromatid cohesion
- Sister chromatid separation
- Spindle checkpoint
ASJC Scopus subject areas
- Cell Biology
- Biochemistry
- Molecular Biology
Cite this
Bub1 multitasking in mitosis. / Yu, Hongtao; Tang, Zhanyun.
In: Cell Cycle, Vol. 4, No. 2, 02.2005, p. 262-265.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bub1 multitasking in mitosis
AU - Yu, Hongtao
AU - Tang, Zhanyun
PY - 2005/2
Y1 - 2005/2
N2 - Accurate partition of duplicated genetic material to the daughter cells during mitosis relies on the maintenance of the physical linkage (cohesion) between sister chromatids until their bipolar attachment to the mitotic spindle. In response to a single straying chromatid within a cell, a surveillance mechanism called the spindle checkpoint blocks the ubiquitin ligase activity of the anaphase-promoting complex or cyclosome (APC/C), stabilizes securin (an APC/C substrate and an inhibitor of separase), and delays the activation of separase. This in turn prevents cleavage of cohesin by separase, preserves sister chromatid cohesion, and delays the onset of anaphase. The protein kinase, Bub1, is a key component of the spindle checkpoint. Bub1 has an upstream function in regulating the kinetochore localization of Mad2 and other downstream checkpoint components. In addition, recent biochemical studies have shown that Bub1 directly phosphorylates the APC/C activator, Cdc20, and inhibits APC/C. Finally, Bub1 has a noncheckpoint function at the kinetochores and preserves centromeric cohesion through the MEI-S332/shugoshin family of proteins. Therefore, Bub1 performs multiple tasks in mitosis that ensure the proper inheritance of chromosomes.
AB - Accurate partition of duplicated genetic material to the daughter cells during mitosis relies on the maintenance of the physical linkage (cohesion) between sister chromatids until their bipolar attachment to the mitotic spindle. In response to a single straying chromatid within a cell, a surveillance mechanism called the spindle checkpoint blocks the ubiquitin ligase activity of the anaphase-promoting complex or cyclosome (APC/C), stabilizes securin (an APC/C substrate and an inhibitor of separase), and delays the activation of separase. This in turn prevents cleavage of cohesin by separase, preserves sister chromatid cohesion, and delays the onset of anaphase. The protein kinase, Bub1, is a key component of the spindle checkpoint. Bub1 has an upstream function in regulating the kinetochore localization of Mad2 and other downstream checkpoint components. In addition, recent biochemical studies have shown that Bub1 directly phosphorylates the APC/C activator, Cdc20, and inhibits APC/C. Finally, Bub1 has a noncheckpoint function at the kinetochores and preserves centromeric cohesion through the MEI-S332/shugoshin family of proteins. Therefore, Bub1 performs multiple tasks in mitosis that ensure the proper inheritance of chromosomes.
KW - Anaphase-promoting complex
KW - Bub1
KW - Cdc20
KW - Cohesin
KW - Cyclosome
KW - MEI-S332
KW - Mitosis
KW - Shugoshin
KW - Sister chromatid cohesion
KW - Sister chromatid separation
KW - Spindle checkpoint
UR - http://www.scopus.com/inward/record.url?scp=21544443242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21544443242&partnerID=8YFLogxK
M3 - Article
C2 - 15655378
AN - SCOPUS:21544443242
VL - 4
SP - 262
EP - 265
JO - Cell Cycle
JF - Cell Cycle
SN - 1538-4101
IS - 2
ER -