Bub1 multitasking in mitosis

Hongtao Yu, Zhanyun Tang

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Accurate partition of duplicated genetic material to the daughter cells during mitosis relies on the maintenance of the physical linkage (cohesion) between sister chromatids until their bipolar attachment to the mitotic spindle. In response to a single straying chromatid within a cell, a surveillance mechanism called the spindle checkpoint blocks the ubiquitin ligase activity of the anaphase-promoting complex or cyclosome (APC/C), stabilizes securin (an APC/C substrate and an inhibitor of separase), and delays the activation of separase. This in turn prevents cleavage of cohesin by separase, preserves sister chromatid cohesion, and delays the onset of anaphase. The protein kinase, Bub1, is a key component of the spindle checkpoint. Bub1 has an upstream function in regulating the kinetochore localization of Mad2 and other downstream checkpoint components. In addition, recent biochemical studies have shown that Bub1 directly phosphorylates the APC/C activator, Cdc20, and inhibits APC/C. Finally, Bub1 has a noncheckpoint function at the kinetochores and preserves centromeric cohesion through the MEI-S332/shugoshin family of proteins. Therefore, Bub1 performs multiple tasks in mitosis that ensure the proper inheritance of chromosomes.

Original languageEnglish (US)
Pages (from-to)262-265
Number of pages4
JournalCell Cycle
Volume4
Issue number2
StatePublished - Feb 2005

Fingerprint

Separase
Anaphase-Promoting Complex-Cyclosome
Multitasking
Chromatids
Mitosis
Kinetochores
Securin
Anaphase
Spindle Apparatus
Ligases
Chromosomes
Ubiquitin
Protein Kinases
Chemical activation
Maintenance
Substrates
Genes
Proteins

Keywords

  • Anaphase-promoting complex
  • Bub1
  • Cdc20
  • Cohesin
  • Cyclosome
  • MEI-S332
  • Mitosis
  • Shugoshin
  • Sister chromatid cohesion
  • Sister chromatid separation
  • Spindle checkpoint

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Cite this

Yu, H., & Tang, Z. (2005). Bub1 multitasking in mitosis. Cell Cycle, 4(2), 262-265.

Bub1 multitasking in mitosis. / Yu, Hongtao; Tang, Zhanyun.

In: Cell Cycle, Vol. 4, No. 2, 02.2005, p. 262-265.

Research output: Contribution to journalArticle

Yu, H & Tang, Z 2005, 'Bub1 multitasking in mitosis', Cell Cycle, vol. 4, no. 2, pp. 262-265.
Yu H, Tang Z. Bub1 multitasking in mitosis. Cell Cycle. 2005 Feb;4(2):262-265.
Yu, Hongtao ; Tang, Zhanyun. / Bub1 multitasking in mitosis. In: Cell Cycle. 2005 ; Vol. 4, No. 2. pp. 262-265.
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