Building the heart piece by piece: Modularity of cis-elements regulating Nkx2-5 transcription

Robert J. Schwartz, Eric N. Olson

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Heart formation in Drosophila is dependent on the homeobox gene tinman. The homeobox gene Nkx2-5 is closely related to tinman and is the earliest known marker for cardiogenesis in vertebrate embryos. Recent studies of cis-regulatory elements required for Nkx2-5 expression in the developing mouse heart have revealed an extraordinary array of independent cardiac enhancers, and associated negative regulatory elements, that direct transcription in distinct regions of the embryonic heart. These studies demonstrate the modularity in cardiac transcription, in which different regulatory elements respond to distinct sets of transcription factors to control gene expression in different compartments of the developing heart. We consider the potential mechanisms underlying such transcriptional complexity, its possible significance for cardiac function, and the implications for evolution of the multichambered heart.

Original languageEnglish (US)
Pages (from-to)4187-4192
Number of pages6
JournalDevelopment
Volume126
Issue number19
StatePublished - Oct 1999

Fingerprint

Homeobox Genes
Drosophila
Vertebrates
Transcription Factors
Embryonic Structures
Gene Expression

Keywords

  • cis-element
  • Heart
  • Nkx2.5
  • Transcription

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Building the heart piece by piece : Modularity of cis-elements regulating Nkx2-5 transcription. / Schwartz, Robert J.; Olson, Eric N.

In: Development, Vol. 126, No. 19, 10.1999, p. 4187-4192.

Research output: Contribution to journalArticle

@article{4fab77aaa44443aeb41929efb510192d,
title = "Building the heart piece by piece: Modularity of cis-elements regulating Nkx2-5 transcription",
abstract = "Heart formation in Drosophila is dependent on the homeobox gene tinman. The homeobox gene Nkx2-5 is closely related to tinman and is the earliest known marker for cardiogenesis in vertebrate embryos. Recent studies of cis-regulatory elements required for Nkx2-5 expression in the developing mouse heart have revealed an extraordinary array of independent cardiac enhancers, and associated negative regulatory elements, that direct transcription in distinct regions of the embryonic heart. These studies demonstrate the modularity in cardiac transcription, in which different regulatory elements respond to distinct sets of transcription factors to control gene expression in different compartments of the developing heart. We consider the potential mechanisms underlying such transcriptional complexity, its possible significance for cardiac function, and the implications for evolution of the multichambered heart.",
keywords = "cis-element, Heart, Nkx2.5, Transcription",
author = "Schwartz, {Robert J.} and Olson, {Eric N.}",
year = "1999",
month = "10",
language = "English (US)",
volume = "126",
pages = "4187--4192",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "19",

}

TY - JOUR

T1 - Building the heart piece by piece

T2 - Modularity of cis-elements regulating Nkx2-5 transcription

AU - Schwartz, Robert J.

AU - Olson, Eric N.

PY - 1999/10

Y1 - 1999/10

N2 - Heart formation in Drosophila is dependent on the homeobox gene tinman. The homeobox gene Nkx2-5 is closely related to tinman and is the earliest known marker for cardiogenesis in vertebrate embryos. Recent studies of cis-regulatory elements required for Nkx2-5 expression in the developing mouse heart have revealed an extraordinary array of independent cardiac enhancers, and associated negative regulatory elements, that direct transcription in distinct regions of the embryonic heart. These studies demonstrate the modularity in cardiac transcription, in which different regulatory elements respond to distinct sets of transcription factors to control gene expression in different compartments of the developing heart. We consider the potential mechanisms underlying such transcriptional complexity, its possible significance for cardiac function, and the implications for evolution of the multichambered heart.

AB - Heart formation in Drosophila is dependent on the homeobox gene tinman. The homeobox gene Nkx2-5 is closely related to tinman and is the earliest known marker for cardiogenesis in vertebrate embryos. Recent studies of cis-regulatory elements required for Nkx2-5 expression in the developing mouse heart have revealed an extraordinary array of independent cardiac enhancers, and associated negative regulatory elements, that direct transcription in distinct regions of the embryonic heart. These studies demonstrate the modularity in cardiac transcription, in which different regulatory elements respond to distinct sets of transcription factors to control gene expression in different compartments of the developing heart. We consider the potential mechanisms underlying such transcriptional complexity, its possible significance for cardiac function, and the implications for evolution of the multichambered heart.

KW - cis-element

KW - Heart

KW - Nkx2.5

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=0032727374&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032727374&partnerID=8YFLogxK

M3 - Article

C2 - 10477287

AN - SCOPUS:0032727374

VL - 126

SP - 4187

EP - 4192

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 19

ER -