Bulla gouldiana period exhibits unique regulation at the mRNA and protein levels

Cara M. Constance, Carla B. Green, Hajime Tei, Gene D. Block

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The authors cloned the period (per) gene from the marine mollusk Bulla gouldiana, a well-characterized circadian model system. This allowed them to examine the characteristics of the per gene in a new phylum, and to make comparisons with the conserved PER domains previously characterized in insects and vertebrates. Only one copy of the per gene is present in the Bulla genome, and it is most similar to PER in two insects: the cockroach, Periplaneta americana, and silkmoth, Antheraea pernyi. Comparison with Drosophila PER (dPER) and murine PER 1 (mPER1) sequence reveals that there is greater sequence homology between Bulla PER (bPER) and dPER in the regions of dPER shown to be important to heterodimerization between dPER and Drosophila timeless. Although the structure suggests conservation between dPER and bPER, expression patterns differ. In all cells and tissues examined that are peripheral to the clock neurons in Bulla, bPer mRNA and protein are expressed constitutively in light:dark (LD) cycles. In the identified clock neurons, the basal retinal neurons (BRNs), a rhythm in bPer expression could be detected in LD cycles with a peak at zeitgeber time (ZT) 5 and trough expression at ZT 13. This temporal profile of expression more closely resembles that of mPER1 than that of dPER. bPer rhythms in the BRNs were not detected in continuous darkness. These analyses suggest that clock genes may be uniquely regulated in different circadian systems, but lead to similar control of rhythms at the cellular, tissue, and organismal levels.

Original languageEnglish (US)
Pages (from-to)413-427
Number of pages15
JournalJournal of Biological Rhythms
Volume17
Issue number5
DOIs
StatePublished - Oct 1 2002

Fingerprint

Blister
Drosophila
Messenger RNA
Proteins
Retinal Neurons
Photoperiod
Genes
Insects
Periplaneta
Neurons
Cockroaches
Bombyx
Moths
Mollusca
Darkness
Sequence Homology
Vertebrates
Genome

Keywords

  • Circadian rhythm
  • Clock
  • Mollusk
  • Retina

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Bulla gouldiana period exhibits unique regulation at the mRNA and protein levels. / Constance, Cara M.; Green, Carla B.; Tei, Hajime; Block, Gene D.

In: Journal of Biological Rhythms, Vol. 17, No. 5, 01.10.2002, p. 413-427.

Research output: Contribution to journalArticle

Constance, Cara M. ; Green, Carla B. ; Tei, Hajime ; Block, Gene D. / Bulla gouldiana period exhibits unique regulation at the mRNA and protein levels. In: Journal of Biological Rhythms. 2002 ; Vol. 17, No. 5. pp. 413-427.
@article{1ccb503e04ac498385d66c9068f4cca0,
title = "Bulla gouldiana period exhibits unique regulation at the mRNA and protein levels",
abstract = "The authors cloned the period (per) gene from the marine mollusk Bulla gouldiana, a well-characterized circadian model system. This allowed them to examine the characteristics of the per gene in a new phylum, and to make comparisons with the conserved PER domains previously characterized in insects and vertebrates. Only one copy of the per gene is present in the Bulla genome, and it is most similar to PER in two insects: the cockroach, Periplaneta americana, and silkmoth, Antheraea pernyi. Comparison with Drosophila PER (dPER) and murine PER 1 (mPER1) sequence reveals that there is greater sequence homology between Bulla PER (bPER) and dPER in the regions of dPER shown to be important to heterodimerization between dPER and Drosophila timeless. Although the structure suggests conservation between dPER and bPER, expression patterns differ. In all cells and tissues examined that are peripheral to the clock neurons in Bulla, bPer mRNA and protein are expressed constitutively in light:dark (LD) cycles. In the identified clock neurons, the basal retinal neurons (BRNs), a rhythm in bPer expression could be detected in LD cycles with a peak at zeitgeber time (ZT) 5 and trough expression at ZT 13. This temporal profile of expression more closely resembles that of mPER1 than that of dPER. bPer rhythms in the BRNs were not detected in continuous darkness. These analyses suggest that clock genes may be uniquely regulated in different circadian systems, but lead to similar control of rhythms at the cellular, tissue, and organismal levels.",
keywords = "Circadian rhythm, Clock, Mollusk, Retina",
author = "Constance, {Cara M.} and Green, {Carla B.} and Hajime Tei and Block, {Gene D.}",
year = "2002",
month = "10",
day = "1",
doi = "10.1177/074873002237136",
language = "English (US)",
volume = "17",
pages = "413--427",
journal = "Journal of Biological Rhythms",
issn = "0748-7304",
publisher = "SAGE Publications Inc.",
number = "5",

}

TY - JOUR

T1 - Bulla gouldiana period exhibits unique regulation at the mRNA and protein levels

AU - Constance, Cara M.

AU - Green, Carla B.

AU - Tei, Hajime

AU - Block, Gene D.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - The authors cloned the period (per) gene from the marine mollusk Bulla gouldiana, a well-characterized circadian model system. This allowed them to examine the characteristics of the per gene in a new phylum, and to make comparisons with the conserved PER domains previously characterized in insects and vertebrates. Only one copy of the per gene is present in the Bulla genome, and it is most similar to PER in two insects: the cockroach, Periplaneta americana, and silkmoth, Antheraea pernyi. Comparison with Drosophila PER (dPER) and murine PER 1 (mPER1) sequence reveals that there is greater sequence homology between Bulla PER (bPER) and dPER in the regions of dPER shown to be important to heterodimerization between dPER and Drosophila timeless. Although the structure suggests conservation between dPER and bPER, expression patterns differ. In all cells and tissues examined that are peripheral to the clock neurons in Bulla, bPer mRNA and protein are expressed constitutively in light:dark (LD) cycles. In the identified clock neurons, the basal retinal neurons (BRNs), a rhythm in bPer expression could be detected in LD cycles with a peak at zeitgeber time (ZT) 5 and trough expression at ZT 13. This temporal profile of expression more closely resembles that of mPER1 than that of dPER. bPer rhythms in the BRNs were not detected in continuous darkness. These analyses suggest that clock genes may be uniquely regulated in different circadian systems, but lead to similar control of rhythms at the cellular, tissue, and organismal levels.

AB - The authors cloned the period (per) gene from the marine mollusk Bulla gouldiana, a well-characterized circadian model system. This allowed them to examine the characteristics of the per gene in a new phylum, and to make comparisons with the conserved PER domains previously characterized in insects and vertebrates. Only one copy of the per gene is present in the Bulla genome, and it is most similar to PER in two insects: the cockroach, Periplaneta americana, and silkmoth, Antheraea pernyi. Comparison with Drosophila PER (dPER) and murine PER 1 (mPER1) sequence reveals that there is greater sequence homology between Bulla PER (bPER) and dPER in the regions of dPER shown to be important to heterodimerization between dPER and Drosophila timeless. Although the structure suggests conservation between dPER and bPER, expression patterns differ. In all cells and tissues examined that are peripheral to the clock neurons in Bulla, bPer mRNA and protein are expressed constitutively in light:dark (LD) cycles. In the identified clock neurons, the basal retinal neurons (BRNs), a rhythm in bPer expression could be detected in LD cycles with a peak at zeitgeber time (ZT) 5 and trough expression at ZT 13. This temporal profile of expression more closely resembles that of mPER1 than that of dPER. bPer rhythms in the BRNs were not detected in continuous darkness. These analyses suggest that clock genes may be uniquely regulated in different circadian systems, but lead to similar control of rhythms at the cellular, tissue, and organismal levels.

KW - Circadian rhythm

KW - Clock

KW - Mollusk

KW - Retina

UR - http://www.scopus.com/inward/record.url?scp=85047686664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047686664&partnerID=8YFLogxK

U2 - 10.1177/074873002237136

DO - 10.1177/074873002237136

M3 - Article

C2 - 12375618

AN - SCOPUS:85047686664

VL - 17

SP - 413

EP - 427

JO - Journal of Biological Rhythms

JF - Journal of Biological Rhythms

SN - 0748-7304

IS - 5

ER -