Bundle-forming pili and EspA are involved in biofilm formation by enteropathogenic Escherichia coli

Cristiano G. Moreira, Kelli Palmer, Marvin Whiteley, Marcelo P. Sircili, Luiz R. Trabulsi, Antonio F P Castro, Vanessa Sperandio

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Abstract

Microcolony formation is one of the initial steps in biofilm development, and in enteropathogenic Escherichia coli (EPEC) it is mediated by several adhesins, including the bundle-forming pilus (BFP) and the EspA filament. Here we report that EPEC forms biofilms on plastic under static conditions and a flowthrough continuous culture system. The abilities of several EPEC isogenic mutants to form biofilms were assessed. Adhesins such as BFP and EspA, important in microcolony formation on epithelial cells, are also involved in bacterial aggregation during biofilm formation on abiotic surfaces. Mutants that do not express BFP or EspA form more-diffuse biofilms than does the wild type. We also determined, using gfp transcriptional fusions, that, consistent with the role of these adhesins in biofilms, the genes encoding BFP and EspA are expressed during biofilm formation. Finally, expression of espA is controlled by a quorum-sensing (QS) regulatory mechanism, and the EPEC qseA QS mutant also forms altered biofilms, suggesting that this signaling mechanism plays an important role in EPEC biofilm development. Taken together, these studies allowed us to propose a model of EPEC biofilm formation.

Original languageEnglish (US)
Pages (from-to)3952-3961
Number of pages10
JournalJournal of Bacteriology
Volume188
Issue number11
DOIs
StatePublished - Jun 2006

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Enteropathogenic Escherichia coli
Biofilms
Quorum Sensing
Plastics
Epithelial Cells

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Moreira, C. G., Palmer, K., Whiteley, M., Sircili, M. P., Trabulsi, L. R., Castro, A. F. P., & Sperandio, V. (2006). Bundle-forming pili and EspA are involved in biofilm formation by enteropathogenic Escherichia coli. Journal of Bacteriology, 188(11), 3952-3961. https://doi.org/10.1128/JB.00177-06

Bundle-forming pili and EspA are involved in biofilm formation by enteropathogenic Escherichia coli. / Moreira, Cristiano G.; Palmer, Kelli; Whiteley, Marvin; Sircili, Marcelo P.; Trabulsi, Luiz R.; Castro, Antonio F P; Sperandio, Vanessa.

In: Journal of Bacteriology, Vol. 188, No. 11, 06.2006, p. 3952-3961.

Research output: Contribution to journalArticle

Moreira, CG, Palmer, K, Whiteley, M, Sircili, MP, Trabulsi, LR, Castro, AFP & Sperandio, V 2006, 'Bundle-forming pili and EspA are involved in biofilm formation by enteropathogenic Escherichia coli', Journal of Bacteriology, vol. 188, no. 11, pp. 3952-3961. https://doi.org/10.1128/JB.00177-06
Moreira CG, Palmer K, Whiteley M, Sircili MP, Trabulsi LR, Castro AFP et al. Bundle-forming pili and EspA are involved in biofilm formation by enteropathogenic Escherichia coli. Journal of Bacteriology. 2006 Jun;188(11):3952-3961. https://doi.org/10.1128/JB.00177-06
Moreira, Cristiano G. ; Palmer, Kelli ; Whiteley, Marvin ; Sircili, Marcelo P. ; Trabulsi, Luiz R. ; Castro, Antonio F P ; Sperandio, Vanessa. / Bundle-forming pili and EspA are involved in biofilm formation by enteropathogenic Escherichia coli. In: Journal of Bacteriology. 2006 ; Vol. 188, No. 11. pp. 3952-3961.
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