C. elegans TCF protein, POP-1, converts from repressor to activator as a result of Wnt-induced lowering of nuclear levels

Premnath Shetty, Miao Chia Lo, Scott M. Robertson, Rueyling Lin

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Canonical Wnt signaling converts the TCF/LEF transcription factor from repressor to activator by increasing nuclear levels of its coactivator, β-catenin. A striking exception had been reported for Wnt-induced endoderm formation during C. elegans embryogenesis. It has long been believed that transcriptional activation of Wnt target genes in the endoderm precursor occurred due to a lowering of nuclear levels of the worm TCF/LEF protein, POP-1, effectively alleviating POP-1 repressive activity. Contrary to this model, we demonstrate here that POP-1 directly activates Wnt target genes in the endoderm precursor. Wnt converts POP-1 from a repressor to an activator, and this conversion requires that POP-1 nuclear levels be lowered in the endoderm precursor. We propose that the balance between TCF/LEF and coactivator(s), achieved by elevating coactivator levels (the canonical pathway) and/or reducing TCF/LEF levels (worm endoderm), determines Wnt signal strength.

Original languageEnglish (US)
Pages (from-to)584-592
Number of pages9
JournalDevelopmental Biology
Volume285
Issue number2
DOIs
StatePublished - Sep 15 2005

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Caenorhabditis elegans Proteins
Endoderm
TCF Transcription Factors
Catenins
Transcriptional Activation
Genes
Embryonic Development
Proteins

Keywords

  • β-catenin
  • C. elegans
  • Endoderm
  • POP-1
  • TCF/LEF
  • Wnt

ASJC Scopus subject areas

  • Developmental Biology

Cite this

C. elegans TCF protein, POP-1, converts from repressor to activator as a result of Wnt-induced lowering of nuclear levels. / Shetty, Premnath; Lo, Miao Chia; Robertson, Scott M.; Lin, Rueyling.

In: Developmental Biology, Vol. 285, No. 2, 15.09.2005, p. 584-592.

Research output: Contribution to journalArticle

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