C-kit-kinase induces a cascade of protein tyrosine phosphorylation in normal human melanocytes in response to mast cell growth factor and stimulates mitogen-activated protein kinase but is down-regulated in melanomas

Yoko Funasaka, Teri Boulton, Melanie Cobb, Yosef Yarden, Baoling Fan, Stewart D. Lyman, Douglas E. Williams, Dirk M. Anderson, Rina Zakut, Yutaka Mishima, Ruth Halaban

Research output: Contribution to journalArticle

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Abstract

The proto-oncogene c-Kit, a transmembrane receptor tyrosine kinase, is an important regulator of cell growth whose constitutively active oncogenic counterpart, v-kit, induces sarcomas in cats. Mutations in murine c-kit that reduce the receptor tyrosine kinase activity cause deficiencies in the migration and proliferation of melanoblasts, hematopoietic stem cells, and primordial germ cells. We therefore investigated whether c-Kit regulates normal human melanocyte proliferation and plays a role in melanomas. We show that normal human melanocytes respond to mast cell growth factor (MGF), the Kit-ligand that stimulates phosphorylation of tyrosyl residues in c-Kit and induces sequential phosphorylation of tyrosyl residues in several other proteins. One of the phosphorylated intermediates in the signal transduction pathway was identified as an early response kinase (mitogen-activated protein [MAP] kinase). Dephosphorylation of a prominent 180-kDa protein suggests that MGF also activates a phosphotyrosine phosphatase. In contrast, MGF did not induce proliferation, the cascade of protein phosphorylations, or MAP kinase activation in the majority of cells cultured from primary nodular and metastatic melanomas that grow independently of exogenous factors. In the five out of eight human melanoma lines expressing c-kit mRNAs, c-Kit was not constitutively activated. Therefore, although c-Kit-kinase is a potent growth regulator of normal human melanocytes, its activity is not positively associated with malignant transformation.

Original languageEnglish (US)
Pages (from-to)197-209
Number of pages13
JournalMolecular Biology of the Cell
Volume3
Issue number2
StatePublished - 1992

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Stem Cell Factor
Melanocytes
Mitogen-Activated Protein Kinases
Tyrosine
Melanoma
Phosphotransferases
Phosphorylation
Proteins
Proto-Oncogene Proteins c-kit
Protein Tyrosine Phosphatases
Proto-Oncogenes
Receptor Protein-Tyrosine Kinases
Growth
Hematopoietic Stem Cells
Germ Cells
Sarcoma
Protein-Tyrosine Kinases
Cultured Cells
Signal Transduction
Cats

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

C-kit-kinase induces a cascade of protein tyrosine phosphorylation in normal human melanocytes in response to mast cell growth factor and stimulates mitogen-activated protein kinase but is down-regulated in melanomas. / Funasaka, Yoko; Boulton, Teri; Cobb, Melanie; Yarden, Yosef; Fan, Baoling; Lyman, Stewart D.; Williams, Douglas E.; Anderson, Dirk M.; Zakut, Rina; Mishima, Yutaka; Halaban, Ruth.

In: Molecular Biology of the Cell, Vol. 3, No. 2, 1992, p. 197-209.

Research output: Contribution to journalArticle

Funasaka, Yoko ; Boulton, Teri ; Cobb, Melanie ; Yarden, Yosef ; Fan, Baoling ; Lyman, Stewart D. ; Williams, Douglas E. ; Anderson, Dirk M. ; Zakut, Rina ; Mishima, Yutaka ; Halaban, Ruth. / C-kit-kinase induces a cascade of protein tyrosine phosphorylation in normal human melanocytes in response to mast cell growth factor and stimulates mitogen-activated protein kinase but is down-regulated in melanomas. In: Molecular Biology of the Cell. 1992 ; Vol. 3, No. 2. pp. 197-209.
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abstract = "The proto-oncogene c-Kit, a transmembrane receptor tyrosine kinase, is an important regulator of cell growth whose constitutively active oncogenic counterpart, v-kit, induces sarcomas in cats. Mutations in murine c-kit that reduce the receptor tyrosine kinase activity cause deficiencies in the migration and proliferation of melanoblasts, hematopoietic stem cells, and primordial germ cells. We therefore investigated whether c-Kit regulates normal human melanocyte proliferation and plays a role in melanomas. We show that normal human melanocytes respond to mast cell growth factor (MGF), the Kit-ligand that stimulates phosphorylation of tyrosyl residues in c-Kit and induces sequential phosphorylation of tyrosyl residues in several other proteins. One of the phosphorylated intermediates in the signal transduction pathway was identified as an early response kinase (mitogen-activated protein [MAP] kinase). Dephosphorylation of a prominent 180-kDa protein suggests that MGF also activates a phosphotyrosine phosphatase. In contrast, MGF did not induce proliferation, the cascade of protein phosphorylations, or MAP kinase activation in the majority of cells cultured from primary nodular and metastatic melanomas that grow independently of exogenous factors. In the five out of eight human melanoma lines expressing c-kit mRNAs, c-Kit was not constitutively activated. Therefore, although c-Kit-kinase is a potent growth regulator of normal human melanocytes, its activity is not positively associated with malignant transformation.",
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