C-NMR: a simple yet comprehensive method for analysis of intermediary metabolism

F. Mark H Jeffrey, Arun Rajagopal, Craig R. Malloy, A. Dear Sherry

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88 Scopus citations

Abstract

13C-NMR is a particularly attractive tool for metabolic studies because of its inherent simplicity: all labeled products at sufficient concentration may be identified and analysed in a single spectrum. However, the real power behind the approach presented here is the ability to measure groups of individual 13C-isotopomers (isotope isomers). The information that this provides is superior to conventional tracer techniques, allowing a very detailed description of metabolic events. Several examples are given of the value of this convenient and straighforward analysis for some problems of current interest in intermediary metabolism.

Original languageEnglish (US)
Pages (from-to)5-10
Number of pages6
JournalTrends in Biochemical Sciences
Volume16
Issue numberC
DOIs
StatePublished - 1991

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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