C3d,g deposits in inflammatory skin diseases: Use of psoriatic skin as a model of cutaneous inflammation

Nicole Basset-Séguin, Marc Porneuf, Olivier Dereure, Valérie Mils, A. Tesnières, Kim B. Yancey, Jean Jacques Guilhou

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Recent studies in our laboratories have shown that human keratinocytes synthesize and secrete complement components including C3. Moreover, human keratinocytederived C3 is regarded as a potential source of C3d,g, a recently described constituent of the sublamina densa region of normal epidermal basement membrane. Additionally, human keratinocyte-derived C3 may also contribute to epidermal basement membrane deposits of C3 in autoimmune or inflammatory skin disorders. To further our understanding of the specificity and origin of epidermal basement membrane C3 deposits in normal and diseased skin, we have characterized in situ deposits of C3 and C3 cleavage fragments in various inflammatory skin diseases and utilized a skin equivalent model to assess the deposition of C3 cleavage fragments in neo-basement membrane of epidermal outgrowths from normal or diseased human skin. C3d,g reactivity was found to be greater in all samples of inflamed skin, and typically associated with C3c reactivity at these sites. No immunoglobulins or other complement components were detected. When lesional psoriatic skin rich in epidermal basement membrane C3c was used in our organ culture system, C3 incorporation within neo-basement membrane was observed. These results show that human keratinocyte-derived C3 may contribute to inflammatory reactions in skin as well as account for deposits of C3d,g in normal epidermal basement membrane.

Original languageEnglish (US)
Pages (from-to)827-831
Number of pages5
JournalJournal of Investigative Dermatology
Volume101
Issue number6
DOIs
StatePublished - Dec 1993

Keywords

  • basement membrane
  • complement
  • psoriasis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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