C3d,g deposits in inflammatory skin diseases: Use of psoriatic skin as a model of cutaneous inflammation

Nicole Basset-Séguin, Marc Porneuf, Olivier Dereure, Valérie Mils, A. Tesnières, Kim B. Yancey, Jean Jacques Guilhou

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recent studies in our laboratories have shown that human keratinocytes synthesize and secrete complement components including C3. Moreover, human keratinocytederived C3 is regarded as a potential source of C3d,g, a recently described constituent of the sublamina densa region of normal epidermal basement membrane. Additionally, human keratinocyte-derived C3 may also contribute to epidermal basement membrane deposits of C3 in autoimmune or inflammatory skin disorders. To further our understanding of the specificity and origin of epidermal basement membrane C3 deposits in normal and diseased skin, we have characterized in situ deposits of C3 and C3 cleavage fragments in various inflammatory skin diseases and utilized a skin equivalent model to assess the deposition of C3 cleavage fragments in neo-basement membrane of epidermal outgrowths from normal or diseased human skin. C3d,g reactivity was found to be greater in all samples of inflamed skin, and typically associated with C3c reactivity at these sites. No immunoglobulins or other complement components were detected. When lesional psoriatic skin rich in epidermal basement membrane C3c was used in our organ culture system, C3 incorporation within neo-basement membrane was observed. These results show that human keratinocyte-derived C3 may contribute to inflammatory reactions in skin as well as account for deposits of C3d,g in normal epidermal basement membrane.

Original languageEnglish (US)
Pages (from-to)827-831
Number of pages5
JournalJournal of Investigative Dermatology
Volume101
Issue number6
StatePublished - Dec 1993

Fingerprint

Skin Diseases
Basement Membrane
Skin
Deposits
Inflammation
Keratinocytes
Command and control systems
Organ Culture Techniques
complement C3d,g
Immunoglobulins

Keywords

  • basement membrane
  • complement
  • psoriasis

ASJC Scopus subject areas

  • Dermatology

Cite this

Basset-Séguin, N., Porneuf, M., Dereure, O., Mils, V., Tesnières, A., Yancey, K. B., & Guilhou, J. J. (1993). C3d,g deposits in inflammatory skin diseases: Use of psoriatic skin as a model of cutaneous inflammation. Journal of Investigative Dermatology, 101(6), 827-831.

C3d,g deposits in inflammatory skin diseases : Use of psoriatic skin as a model of cutaneous inflammation. / Basset-Séguin, Nicole; Porneuf, Marc; Dereure, Olivier; Mils, Valérie; Tesnières, A.; Yancey, Kim B.; Guilhou, Jean Jacques.

In: Journal of Investigative Dermatology, Vol. 101, No. 6, 12.1993, p. 827-831.

Research output: Contribution to journalArticle

Basset-Séguin, N, Porneuf, M, Dereure, O, Mils, V, Tesnières, A, Yancey, KB & Guilhou, JJ 1993, 'C3d,g deposits in inflammatory skin diseases: Use of psoriatic skin as a model of cutaneous inflammation', Journal of Investigative Dermatology, vol. 101, no. 6, pp. 827-831.
Basset-Séguin, Nicole ; Porneuf, Marc ; Dereure, Olivier ; Mils, Valérie ; Tesnières, A. ; Yancey, Kim B. ; Guilhou, Jean Jacques. / C3d,g deposits in inflammatory skin diseases : Use of psoriatic skin as a model of cutaneous inflammation. In: Journal of Investigative Dermatology. 1993 ; Vol. 101, No. 6. pp. 827-831.
@article{fcf5a0ececf340a6b29fecda81409078,
title = "C3d,g deposits in inflammatory skin diseases: Use of psoriatic skin as a model of cutaneous inflammation",
abstract = "Recent studies in our laboratories have shown that human keratinocytes synthesize and secrete complement components including C3. Moreover, human keratinocytederived C3 is regarded as a potential source of C3d,g, a recently described constituent of the sublamina densa region of normal epidermal basement membrane. Additionally, human keratinocyte-derived C3 may also contribute to epidermal basement membrane deposits of C3 in autoimmune or inflammatory skin disorders. To further our understanding of the specificity and origin of epidermal basement membrane C3 deposits in normal and diseased skin, we have characterized in situ deposits of C3 and C3 cleavage fragments in various inflammatory skin diseases and utilized a skin equivalent model to assess the deposition of C3 cleavage fragments in neo-basement membrane of epidermal outgrowths from normal or diseased human skin. C3d,g reactivity was found to be greater in all samples of inflamed skin, and typically associated with C3c reactivity at these sites. No immunoglobulins or other complement components were detected. When lesional psoriatic skin rich in epidermal basement membrane C3c was used in our organ culture system, C3 incorporation within neo-basement membrane was observed. These results show that human keratinocyte-derived C3 may contribute to inflammatory reactions in skin as well as account for deposits of C3d,g in normal epidermal basement membrane.",
keywords = "basement membrane, complement, psoriasis",
author = "Nicole Basset-S{\'e}guin and Marc Porneuf and Olivier Dereure and Val{\'e}rie Mils and A. Tesni{\`e}res and Yancey, {Kim B.} and Guilhou, {Jean Jacques}",
year = "1993",
month = "12",
language = "English (US)",
volume = "101",
pages = "827--831",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - C3d,g deposits in inflammatory skin diseases

T2 - Use of psoriatic skin as a model of cutaneous inflammation

AU - Basset-Séguin, Nicole

AU - Porneuf, Marc

AU - Dereure, Olivier

AU - Mils, Valérie

AU - Tesnières, A.

AU - Yancey, Kim B.

AU - Guilhou, Jean Jacques

PY - 1993/12

Y1 - 1993/12

N2 - Recent studies in our laboratories have shown that human keratinocytes synthesize and secrete complement components including C3. Moreover, human keratinocytederived C3 is regarded as a potential source of C3d,g, a recently described constituent of the sublamina densa region of normal epidermal basement membrane. Additionally, human keratinocyte-derived C3 may also contribute to epidermal basement membrane deposits of C3 in autoimmune or inflammatory skin disorders. To further our understanding of the specificity and origin of epidermal basement membrane C3 deposits in normal and diseased skin, we have characterized in situ deposits of C3 and C3 cleavage fragments in various inflammatory skin diseases and utilized a skin equivalent model to assess the deposition of C3 cleavage fragments in neo-basement membrane of epidermal outgrowths from normal or diseased human skin. C3d,g reactivity was found to be greater in all samples of inflamed skin, and typically associated with C3c reactivity at these sites. No immunoglobulins or other complement components were detected. When lesional psoriatic skin rich in epidermal basement membrane C3c was used in our organ culture system, C3 incorporation within neo-basement membrane was observed. These results show that human keratinocyte-derived C3 may contribute to inflammatory reactions in skin as well as account for deposits of C3d,g in normal epidermal basement membrane.

AB - Recent studies in our laboratories have shown that human keratinocytes synthesize and secrete complement components including C3. Moreover, human keratinocytederived C3 is regarded as a potential source of C3d,g, a recently described constituent of the sublamina densa region of normal epidermal basement membrane. Additionally, human keratinocyte-derived C3 may also contribute to epidermal basement membrane deposits of C3 in autoimmune or inflammatory skin disorders. To further our understanding of the specificity and origin of epidermal basement membrane C3 deposits in normal and diseased skin, we have characterized in situ deposits of C3 and C3 cleavage fragments in various inflammatory skin diseases and utilized a skin equivalent model to assess the deposition of C3 cleavage fragments in neo-basement membrane of epidermal outgrowths from normal or diseased human skin. C3d,g reactivity was found to be greater in all samples of inflamed skin, and typically associated with C3c reactivity at these sites. No immunoglobulins or other complement components were detected. When lesional psoriatic skin rich in epidermal basement membrane C3c was used in our organ culture system, C3 incorporation within neo-basement membrane was observed. These results show that human keratinocyte-derived C3 may contribute to inflammatory reactions in skin as well as account for deposits of C3d,g in normal epidermal basement membrane.

KW - basement membrane

KW - complement

KW - psoriasis

UR - http://www.scopus.com/inward/record.url?scp=0027515812&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027515812&partnerID=8YFLogxK

M3 - Article

C2 - 8245511

AN - SCOPUS:0027515812

VL - 101

SP - 827

EP - 831

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 6

ER -