C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation

Ying Liu, Xuecheng Ye, Feng Jiang, Chunyang Liang, Dongmei Chen, Junmin Peng, Lisa N. Kinch, Nick V. Grishin, Qinghua Liu

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

The catalytic engine of RNA interference (RNAi) is the RNA-induced silencing complex (RISC), wherein the endoribonuclease Argonaute and single-stranded small interfering RNA (siRNA) direct target mRNA cleavage. We reconstituted long double-stranded RNA- and duplex si RNA-initiated RISC activities with the use of recombinant Drosophila Dicer-2, R2D2, and Ago2 proteins. We used this core reconstitution system to purify an RNAi regulator that we term C3PO (component 3 promoter of RISC), a complex of Translin and Trax. C3PO is a Mg2+-dependent endoribonuclease that promotes RISC activation by removing siRNA passenger strand cleavage products. These studies establish an in vitro RNAi reconstitution system and identify C3PO as a key activator of the core RNAi machinery.

Original languageEnglish (US)
Pages (from-to)750-753
Number of pages4
JournalScience
Volume325
Issue number5941
DOIs
StatePublished - 2009

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RNA-Induced Silencing Complex
Endoribonucleases
RNA Interference
Small Interfering RNA
Catalytic RNA
Double-Stranded RNA
Drosophila
RNA
Messenger RNA
Proteins

ASJC Scopus subject areas

  • General

Cite this

Liu, Y., Ye, X., Jiang, F., Liang, C., Chen, D., Peng, J., ... Liu, Q. (2009). C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation. Science, 325(5941), 750-753. https://doi.org/10.1126/science.1176325

C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation. / Liu, Ying; Ye, Xuecheng; Jiang, Feng; Liang, Chunyang; Chen, Dongmei; Peng, Junmin; Kinch, Lisa N.; Grishin, Nick V.; Liu, Qinghua.

In: Science, Vol. 325, No. 5941, 2009, p. 750-753.

Research output: Contribution to journalArticle

Liu, Y, Ye, X, Jiang, F, Liang, C, Chen, D, Peng, J, Kinch, LN, Grishin, NV & Liu, Q 2009, 'C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation', Science, vol. 325, no. 5941, pp. 750-753. https://doi.org/10.1126/science.1176325
Liu, Ying ; Ye, Xuecheng ; Jiang, Feng ; Liang, Chunyang ; Chen, Dongmei ; Peng, Junmin ; Kinch, Lisa N. ; Grishin, Nick V. ; Liu, Qinghua. / C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation. In: Science. 2009 ; Vol. 325, No. 5941. pp. 750-753.
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