Ca2+-calmodulin-dependent polymerization of actin by myelin basic protein.

Z. Dobrowolski, H. Osińska, M. Mossakowska, B. Baryłko

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The interaction between myelin basic protein (MBP) and G-actin was studied under nonpolymerizing conditions, i.e.,2mM HEPES, pH 7.5, 0.1 mM CaCl2 and 0.2 mM ATP. Fluorescence studies using pyrenyl-actin and the measurements of ATP hydrolysis rate show that MBP induces changes in the structure of the actin monomer similar to those occurring during polymerization by salt. Electron microscope observations of the MBP-G-actin complex reveal the presence of filamentous structures which appear as separate filaments or as bundles of filaments in lateral association. These filaments are polar as visualized by attachment of heavy meromyosin. The biochemical data together with electron microscope observations suggest that the binding of MBP to G-actin under non-polymerizing conditions induces an interaction between actin monomers leading to the formation of filamentous structures which may be similar to F-actin filaments. The effects of MBP on G-actin can be reversed by calmodulin in the presence of Ca2+.

Original languageEnglish (US)
Pages (from-to)17-26
Number of pages10
JournalEuropean Journal of Cell Biology
Volume42
Issue number1
StatePublished - Oct 1986

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Myelin Basic Protein
Calmodulin
Polymerization
Actins
Electrons
HEPES
Myosin Subfragments
Actin Cytoskeleton
Hydrolysis
Salts
Adenosine Triphosphate
Fluorescence

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

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Ca2+-calmodulin-dependent polymerization of actin by myelin basic protein. / Dobrowolski, Z.; Osińska, H.; Mossakowska, M.; Baryłko, B.

In: European Journal of Cell Biology, Vol. 42, No. 1, 10.1986, p. 17-26.

Research output: Contribution to journalArticle

Dobrowolski, Z, Osińska, H, Mossakowska, M & Baryłko, B 1986, 'Ca2+-calmodulin-dependent polymerization of actin by myelin basic protein.', European Journal of Cell Biology, vol. 42, no. 1, pp. 17-26.
Dobrowolski, Z. ; Osińska, H. ; Mossakowska, M. ; Baryłko, B. / Ca2+-calmodulin-dependent polymerization of actin by myelin basic protein. In: European Journal of Cell Biology. 1986 ; Vol. 42, No. 1. pp. 17-26.
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