Cab45, a novel Ca2+-binding protein localized to the Golgi lumen

Philipp E. Scherer, Gerardo Z. Lederkremer, Suzanne Williams, Michael Fogliano, Giulia Baldini, Harvey F. Lodish

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

We have identified and characterized Cab45, a novel 45-kD protein from mouse 3T3-L1 adipocytes. Cab45 is ubiquitously expressed, contains an NH2-terminal signal sequence but no membrane-anchor sequences, and binds Ca2+ due to the presence of six EF-hand motifs. Within the superfamily of calcium-binding proteins, it belongs to a recently identified group of proteins consisting of Reticulocalbin (Ozawa, M., and T. Muramatsu. 1993. J. Biol. Chem. 268:699-705) and ERC 55 (Weis, K., G. Griffiths, and A.I. Lamond. 1994. J. Biol. Chem. 269:19142-19150), both of which share significant sequence homology with Cab45 outside the EF-hand motifs. In contrast to reticulocalbin and ERC-55 which are soluble components of the endoplasmic reticulum, Cab45 is a soluble protein localized to the Golgi. Cab45 is the first calcium-binding protein localized to the lumenal portion of a post-ER compartment; Cab45 is also the first known soluble protein resident in the Golgi lumen. Cab45 can serve as a model protein to determine the mechanism of retention of soluble proteins in the Golgi compartment.

Original languageEnglish (US)
Pages (from-to)257-268
Number of pages12
JournalJournal of Cell Biology
Volume133
Issue number2
StatePublished - Apr 1996

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Carrier Proteins
EF Hand Motifs
Proteins
Calcium-Binding Proteins
Sequence Homology
Protein Sorting Signals
Adipocytes
Endoplasmic Reticulum
Membranes
reticulocalbin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Scherer, P. E., Lederkremer, G. Z., Williams, S., Fogliano, M., Baldini, G., & Lodish, H. F. (1996). Cab45, a novel Ca2+-binding protein localized to the Golgi lumen. Journal of Cell Biology, 133(2), 257-268.

Cab45, a novel Ca2+-binding protein localized to the Golgi lumen. / Scherer, Philipp E.; Lederkremer, Gerardo Z.; Williams, Suzanne; Fogliano, Michael; Baldini, Giulia; Lodish, Harvey F.

In: Journal of Cell Biology, Vol. 133, No. 2, 04.1996, p. 257-268.

Research output: Contribution to journalArticle

Scherer, PE, Lederkremer, GZ, Williams, S, Fogliano, M, Baldini, G & Lodish, HF 1996, 'Cab45, a novel Ca2+-binding protein localized to the Golgi lumen', Journal of Cell Biology, vol. 133, no. 2, pp. 257-268.
Scherer PE, Lederkremer GZ, Williams S, Fogliano M, Baldini G, Lodish HF. Cab45, a novel Ca2+-binding protein localized to the Golgi lumen. Journal of Cell Biology. 1996 Apr;133(2):257-268.
Scherer, Philipp E. ; Lederkremer, Gerardo Z. ; Williams, Suzanne ; Fogliano, Michael ; Baldini, Giulia ; Lodish, Harvey F. / Cab45, a novel Ca2+-binding protein localized to the Golgi lumen. In: Journal of Cell Biology. 1996 ; Vol. 133, No. 2. pp. 257-268.
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