Cachectin/tumor necrosis factor mediates changes of skeletal muscle plasma membrane potential

K. J. Tracey, S. F. Lowry, B. Beutler, A. Cerami, J. D. Albert, G. T. Shires

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Lethal infections are associated with cellular dysfunction as evidenced by a decrease in the resting transmembrane potential difference (E(m)) of skeletal muscle fibers. Endotoxin stimulation of macrophages evokes production of cachectin, a protein that has been implicated as a mediator of the lethal effects of endotoxemia. In the present study, rat skeletal muscle fiber E(m) decreased when incubated with recombinant human cachectin. The reduction of E(m) induced by cachectin occurred in a dose-related fashion and was inhibited by mAb against the monokine. Infusion of cachectin induced a decline of skeletal muscle E(m) in vivo, and suggests that cachectin may acutely mediate alterations of skeletal muscle membrane function after infection.

Original languageEnglish (US)
Pages (from-to)1368-1373
Number of pages6
JournalJournal of Experimental Medicine
Volume164
Issue number4
StatePublished - 1986

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Membrane Potentials
Skeletal Muscle
Tumor Necrosis Factor-alpha
Cell Membrane
Skeletal Muscle Fibers
Monokines
Endotoxemia
Infection
Endotoxins
Macrophages
Membranes
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Tracey, K. J., Lowry, S. F., Beutler, B., Cerami, A., Albert, J. D., & Shires, G. T. (1986). Cachectin/tumor necrosis factor mediates changes of skeletal muscle plasma membrane potential. Journal of Experimental Medicine, 164(4), 1368-1373.

Cachectin/tumor necrosis factor mediates changes of skeletal muscle plasma membrane potential. / Tracey, K. J.; Lowry, S. F.; Beutler, B.; Cerami, A.; Albert, J. D.; Shires, G. T.

In: Journal of Experimental Medicine, Vol. 164, No. 4, 1986, p. 1368-1373.

Research output: Contribution to journalArticle

Tracey, KJ, Lowry, SF, Beutler, B, Cerami, A, Albert, JD & Shires, GT 1986, 'Cachectin/tumor necrosis factor mediates changes of skeletal muscle plasma membrane potential', Journal of Experimental Medicine, vol. 164, no. 4, pp. 1368-1373.
Tracey, K. J. ; Lowry, S. F. ; Beutler, B. ; Cerami, A. ; Albert, J. D. ; Shires, G. T. / Cachectin/tumor necrosis factor mediates changes of skeletal muscle plasma membrane potential. In: Journal of Experimental Medicine. 1986 ; Vol. 164, No. 4. pp. 1368-1373.
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