TY - JOUR
T1 - Calbindin-D(28k) buffers intracellular calcium and promotes resistance to degeneration in PC12 cells
AU - McMahon, Anne
AU - Wong, Brendan S.
AU - Iacopino, Anthony M.
AU - Ng, May C.
AU - Chi, Susan
AU - German, Dwight C.
N1 - Funding Information:
Research supported by NIH grant NS30406, and the Carl J. and Hortense M. Thomsen Chair in Alzheimer's Disease Research. The authors wish to thank Mike Lee for technical assistance, and Judy Burdette for secretarial assistance.
PY - 1998/2
Y1 - 1998/2
N2 - The calcium-binding protein calbindin-D(28k) (CB) has been hypothesized to function, in part, as a neuroprotective protein. CB is localized within nerve cells that are often less vulnerable to degeneration in patients with Alzheimer's disease and Parkinson's disease, and cells containing CB can buffer intracellular calcium concentrations ([Ca2+](i). The present study was designed to directly test the hypothesis that CB can protect cells from degeneration by reducing [Ca2+](i). PC12 cells, transfected to express different levels of CB, were found to be significantly less vulnerable to degeneration caused by serum withdrawal, glutamate, and the neurotoxin 1-methyl-4-phenylpyridinium (MPP+). However, CB did not protect cells from degeneration caused by the calcium ionophore A23187. CB-transfected cells exhibited reduced elevations in [Ca2+](i) following treatment with bradykinin, or ATP compared to non-CB-containing cells. These data indicate that CB can protect cells from degeneration caused by certain conditions, and it reduces elevations in [Ca2+](i) caused by influx from extracellular sources.
AB - The calcium-binding protein calbindin-D(28k) (CB) has been hypothesized to function, in part, as a neuroprotective protein. CB is localized within nerve cells that are often less vulnerable to degeneration in patients with Alzheimer's disease and Parkinson's disease, and cells containing CB can buffer intracellular calcium concentrations ([Ca2+](i). The present study was designed to directly test the hypothesis that CB can protect cells from degeneration by reducing [Ca2+](i). PC12 cells, transfected to express different levels of CB, were found to be significantly less vulnerable to degeneration caused by serum withdrawal, glutamate, and the neurotoxin 1-methyl-4-phenylpyridinium (MPP+). However, CB did not protect cells from degeneration caused by the calcium ionophore A23187. CB-transfected cells exhibited reduced elevations in [Ca2+](i) following treatment with bradykinin, or ATP compared to non-CB-containing cells. These data indicate that CB can protect cells from degeneration caused by certain conditions, and it reduces elevations in [Ca2+](i) caused by influx from extracellular sources.
KW - A23187
KW - Calcium imaging
KW - Glutamate
KW - Serum withdrawal
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U2 - 10.1016/S0169-328X(97)00305-7
DO - 10.1016/S0169-328X(97)00305-7
M3 - Article
C2 - 9526044
AN - SCOPUS:0031910161
SN - 0169-328X
VL - 54
SP - 56
EP - 63
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -