Calcitonin secretion by continuous cultures of small cell carcinoma of the lung: Incidence and immunoheterogeneity studies

Kenneth L. Becker; Adi F. Gazdar; Desmond N. Carney; Richard H. Snider; Charles F. Moore; Omega L. Silva

doi:10.1016/0304-3835(83)90065-4

Calcitonin secretion by continuous cultures of small cell carcinoma of the lung: Incidence and immunoheterogeneity studies

Kenneth L. Becker, Adi F. Gazdar, Desmond N. Carney, Richard H. Snider, Charles F. Moore, Omega L. Silva

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

A study was made of immunoreactive calcitonin (iCT) secretion by continuous cultures of small cell carcinoma of the lung (SCCL). Using an antiserum region specific for the midportion of the molecule, 9 12 cultures were found to secrete iCT. Gel filtration studies were performed on both supernatant fluid (SF) and cell pellet (CP) extract from a culture secreting high levels of iCT. Multiple iCT fractions were found in the SF with the major fraction being of high molecular weight (MW). In contrast, the CP had apparently monomeric CT as its principal iCT fraction. These studies demonstrate frequent iCT secretion by SCCL cultures and significant disparities between the iCT moieties found extra- and intracellularly.

Original language	English (US)
Pages (from-to)	179-185
Number of pages	7
Journal	Cancer Letters
Volume	18
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(83)90065-4
State	Published - Mar 1983

ASJC Scopus subject areas

Oncology
Cancer Research

Access to Document

10.1016/0304-3835(83)90065-4

Cite this

@article{9c1a97850eb840fb9dc1329aeb1e08cb,

title = "Calcitonin secretion by continuous cultures of small cell carcinoma of the lung: Incidence and immunoheterogeneity studies",

abstract = "A study was made of immunoreactive calcitonin (iCT) secretion by continuous cultures of small cell carcinoma of the lung (SCCL). Using an antiserum region specific for the midportion of the molecule, 9 12 cultures were found to secrete iCT. Gel filtration studies were performed on both supernatant fluid (SF) and cell pellet (CP) extract from a culture secreting high levels of iCT. Multiple iCT fractions were found in the SF with the major fraction being of high molecular weight (MW). In contrast, the CP had apparently monomeric CT as its principal iCT fraction. These studies demonstrate frequent iCT secretion by SCCL cultures and significant disparities between the iCT moieties found extra- and intracellularly.",

author = "Becker, {Kenneth L.} and Gazdar, {Adi F.} and Carney, {Desmond N.} and Snider, {Richard H.} and Moore, {Charles F.} and Silva, {Omega L.}",

year = "1983",

month = mar,

doi = "10.1016/0304-3835(83)90065-4",

language = "English (US)",

volume = "18",

pages = "179--185",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Calcitonin secretion by continuous cultures of small cell carcinoma of the lung

T2 - Incidence and immunoheterogeneity studies

AU - Becker, Kenneth L.

AU - Gazdar, Adi F.

AU - Carney, Desmond N.

AU - Snider, Richard H.

AU - Moore, Charles F.

AU - Silva, Omega L.

PY - 1983/3

Y1 - 1983/3

N2 - A study was made of immunoreactive calcitonin (iCT) secretion by continuous cultures of small cell carcinoma of the lung (SCCL). Using an antiserum region specific for the midportion of the molecule, 9 12 cultures were found to secrete iCT. Gel filtration studies were performed on both supernatant fluid (SF) and cell pellet (CP) extract from a culture secreting high levels of iCT. Multiple iCT fractions were found in the SF with the major fraction being of high molecular weight (MW). In contrast, the CP had apparently monomeric CT as its principal iCT fraction. These studies demonstrate frequent iCT secretion by SCCL cultures and significant disparities between the iCT moieties found extra- and intracellularly.

AB - A study was made of immunoreactive calcitonin (iCT) secretion by continuous cultures of small cell carcinoma of the lung (SCCL). Using an antiserum region specific for the midportion of the molecule, 9 12 cultures were found to secrete iCT. Gel filtration studies were performed on both supernatant fluid (SF) and cell pellet (CP) extract from a culture secreting high levels of iCT. Multiple iCT fractions were found in the SF with the major fraction being of high molecular weight (MW). In contrast, the CP had apparently monomeric CT as its principal iCT fraction. These studies demonstrate frequent iCT secretion by SCCL cultures and significant disparities between the iCT moieties found extra- and intracellularly.

UR - http://www.scopus.com/inward/record.url?scp=0020628791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020628791&partnerID=8YFLogxK

U2 - 10.1016/0304-3835(83)90065-4

DO - 10.1016/0304-3835(83)90065-4

M3 - Article

C2 - 6299519

AN - SCOPUS:0020628791

SN - 0304-3835

VL - 18

SP - 179

EP - 185

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Calcitonin secretion by continuous cultures of small cell carcinoma of the lung: Incidence and immunoheterogeneity studies

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this