Calcium‐Dependent Proteases in Neuroblastoma Cells

G. N. DeMartino; D. E. Croall

doi:10.1111/j.1471-4159.1982.tb06645.x

Calcium‐Dependent Proteases in Neuroblastoma Cells

G. N. DeMartino, D. E. Croall

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Abstract: Extracts of neuroblastoma cells contained calcium‐dependent proteolytic activity. This activity was accounted for by two distinct proteases. These proteases were separated by ion‐exchange chromatography. Although each was totally dependent on calcium, the calcium requirements of the enzymes were different; one enzyme required approximately 40 μM Ca²⁺ for half‐maximal activity and the other enzyme required approximately 150 μM Ca²⁺ for half‐maximal activity.

Original language	English (US)
Pages (from-to)	1642-1648
Number of pages	7
Journal	Journal of Neurochemistry
Volume	38
Issue number	6
DOIs	https://doi.org/10.1111/j.1471-4159.1982.tb06645.x
State	Published - Jun 1982

Keywords

Calcium
Neuroblastoma
Neurofilaments
Proteases
Protein degradation

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.1982.tb06645.x

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AB - Abstract: Extracts of neuroblastoma cells contained calcium‐dependent proteolytic activity. This activity was accounted for by two distinct proteases. These proteases were separated by ion‐exchange chromatography. Although each was totally dependent on calcium, the calcium requirements of the enzymes were different; one enzyme required approximately 40 μM Ca2+ for half‐maximal activity and the other enzyme required approximately 150 μM Ca2+ for half‐maximal activity.

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KW - Neurofilaments

KW - Proteases

KW - Protein degradation

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Calcium‐Dependent Proteases in Neuroblastoma Cells

Abstract

Keywords

ASJC Scopus subject areas

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