Calibration and validation of TRUST MRI for the estimation of cerebral blood oxygenation

Hanzhang Lu, Feng Xu, Ksenija Grgac, Peiying Liu, Qin Qin, Peter Van Zijl

Research output: Contribution to journalArticle

110 Scopus citations


Recently, a T 2-Relaxation-Under-Spin-Tagging (TRUST) MRI technique was developed to quantitatively estimate blood oxygen saturation fraction (Y) via the measurement of pure blood T 2. This technique has shown promise for normalization of fMRI signals, for the assessment of oxygen metabolism, and in studies of cognitive aging and multiple sclerosis. However, a human validation study has not been conducted. In addition, the calibration curve used to convert blood T 2 to Y has not accounted for the effects of hematocrit (Hct). In this study, we first conducted experiments on blood samples under physiologic conditions, and the Carr-Purcell-Meiboom-Gill T 2 was determined for a range of Y and Hct values. The data were fitted to a two-compartment exchange model to allow the characterization of a three-dimensional plot that can serve to calibrate the in vivo data. Next, in a validation study in humans, we showed that arterial Y estimated using TRUST MRI was 0.837 ± 0.036 (N=7) during the inhalation of 14% O2, which was in excellent agreement with the gold-standard Y values of 0.840 ± 0.036 based on Pulse-Oximetry. These data suggest that the availability of this calibration plot should enhance the applicability of T 2-Relaxation- Under-Spin-Tagging MRI for noninvasive assessment of cerebral blood oxygenation.

Original languageEnglish (US)
Pages (from-to)42-49
Number of pages8
JournalMagnetic resonance in medicine
Issue number1
StatePublished - Jan 2012


  • T relaxation under spin tagging
  • arterial spin labeling
  • cerebral blood flow
  • hypoxia
  • normoxia

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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