Calorie restriction increases Fas/Fas-ligand expression and apoptosis in murine splenic lymphocytes

C. P. Reddy Avula, A. Muthukumar, G. Fernandes

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

One-month-old male ICR mice were fed a nutritionally adequate, semipurified diet, either ad libitum (AL) or calorie restricted (CR) (40% less food) for 6 months and were killed to obtain spleens. Flow cytometric analysis revealed increased proportions of both CD4+ and CD8+ T cells in CR-fed mice compared to AL-fed mice. The T cell subsets of CR-fed mice were also found to have higher levels of plasma membrane Fas receptor expression. Similarly, Fas-ligand (Fas-L) expression was higher in anti-CD3-stimulated CD4+ and CD8+ T cells. CR-fed mice also had increased numbers of annexin V-positive CD4+ and CD8+ T cells in stimulated splenic lymphocytes suggesting an increased potential for apoptosis. Fas and Fas-L gene expression in splenic lymphocytes, which correlated closely with the observed increased rate of apoptosis, was significantly increased in CR-fed mice compared to AL-fed mice. In conclusion, these results indicate that CR increases the expression of Fas and Fas-L which may contribute to the known beneficial effects of CR such as prolongation of life span by activating chronic physiologically mediated apoptosis. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)231-235
Number of pages5
JournalFEBS Letters
Volume458
Issue number2
DOIs
StatePublished - Sep 17 1999

Fingerprint

Fas Ligand Protein
T-cells
Lymphocytes
Apoptosis
CD95 Antigens
T-Lymphocytes
Annexin A5
Cell membranes
Nutrition
Gene expression
Life Support Care
Inbred ICR Mouse
T-Lymphocyte Subsets
Spleen
Cell Membrane
Diet
Gene Expression
Food

Keywords

  • Apoptosis
  • Calorie restriction
  • Fas (CD95)
  • Fas-ligand (CD95L)
  • Splenic lymphocyte subset

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Calorie restriction increases Fas/Fas-ligand expression and apoptosis in murine splenic lymphocytes. / Reddy Avula, C. P.; Muthukumar, A.; Fernandes, G.

In: FEBS Letters, Vol. 458, No. 2, 17.09.1999, p. 231-235.

Research output: Contribution to journalArticle

@article{d8c222c5607d475586ee16d79b58880f,
title = "Calorie restriction increases Fas/Fas-ligand expression and apoptosis in murine splenic lymphocytes",
abstract = "One-month-old male ICR mice were fed a nutritionally adequate, semipurified diet, either ad libitum (AL) or calorie restricted (CR) (40{\%} less food) for 6 months and were killed to obtain spleens. Flow cytometric analysis revealed increased proportions of both CD4+ and CD8+ T cells in CR-fed mice compared to AL-fed mice. The T cell subsets of CR-fed mice were also found to have higher levels of plasma membrane Fas receptor expression. Similarly, Fas-ligand (Fas-L) expression was higher in anti-CD3-stimulated CD4+ and CD8+ T cells. CR-fed mice also had increased numbers of annexin V-positive CD4+ and CD8+ T cells in stimulated splenic lymphocytes suggesting an increased potential for apoptosis. Fas and Fas-L gene expression in splenic lymphocytes, which correlated closely with the observed increased rate of apoptosis, was significantly increased in CR-fed mice compared to AL-fed mice. In conclusion, these results indicate that CR increases the expression of Fas and Fas-L which may contribute to the known beneficial effects of CR such as prolongation of life span by activating chronic physiologically mediated apoptosis. Copyright (C) 1999 Federation of European Biochemical Societies.",
keywords = "Apoptosis, Calorie restriction, Fas (CD95), Fas-ligand (CD95L), Splenic lymphocyte subset",
author = "{Reddy Avula}, {C. P.} and A. Muthukumar and G. Fernandes",
year = "1999",
month = "9",
day = "17",
doi = "10.1016/S0014-5793(99)01163-1",
language = "English (US)",
volume = "458",
pages = "231--235",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Calorie restriction increases Fas/Fas-ligand expression and apoptosis in murine splenic lymphocytes

AU - Reddy Avula, C. P.

AU - Muthukumar, A.

AU - Fernandes, G.

PY - 1999/9/17

Y1 - 1999/9/17

N2 - One-month-old male ICR mice were fed a nutritionally adequate, semipurified diet, either ad libitum (AL) or calorie restricted (CR) (40% less food) for 6 months and were killed to obtain spleens. Flow cytometric analysis revealed increased proportions of both CD4+ and CD8+ T cells in CR-fed mice compared to AL-fed mice. The T cell subsets of CR-fed mice were also found to have higher levels of plasma membrane Fas receptor expression. Similarly, Fas-ligand (Fas-L) expression was higher in anti-CD3-stimulated CD4+ and CD8+ T cells. CR-fed mice also had increased numbers of annexin V-positive CD4+ and CD8+ T cells in stimulated splenic lymphocytes suggesting an increased potential for apoptosis. Fas and Fas-L gene expression in splenic lymphocytes, which correlated closely with the observed increased rate of apoptosis, was significantly increased in CR-fed mice compared to AL-fed mice. In conclusion, these results indicate that CR increases the expression of Fas and Fas-L which may contribute to the known beneficial effects of CR such as prolongation of life span by activating chronic physiologically mediated apoptosis. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - One-month-old male ICR mice were fed a nutritionally adequate, semipurified diet, either ad libitum (AL) or calorie restricted (CR) (40% less food) for 6 months and were killed to obtain spleens. Flow cytometric analysis revealed increased proportions of both CD4+ and CD8+ T cells in CR-fed mice compared to AL-fed mice. The T cell subsets of CR-fed mice were also found to have higher levels of plasma membrane Fas receptor expression. Similarly, Fas-ligand (Fas-L) expression was higher in anti-CD3-stimulated CD4+ and CD8+ T cells. CR-fed mice also had increased numbers of annexin V-positive CD4+ and CD8+ T cells in stimulated splenic lymphocytes suggesting an increased potential for apoptosis. Fas and Fas-L gene expression in splenic lymphocytes, which correlated closely with the observed increased rate of apoptosis, was significantly increased in CR-fed mice compared to AL-fed mice. In conclusion, these results indicate that CR increases the expression of Fas and Fas-L which may contribute to the known beneficial effects of CR such as prolongation of life span by activating chronic physiologically mediated apoptosis. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Apoptosis

KW - Calorie restriction

KW - Fas (CD95)

KW - Fas-ligand (CD95L)

KW - Splenic lymphocyte subset

UR - http://www.scopus.com/inward/record.url?scp=0032821422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032821422&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(99)01163-1

DO - 10.1016/S0014-5793(99)01163-1

M3 - Article

C2 - 10481071

AN - SCOPUS:0032821422

VL - 458

SP - 231

EP - 235

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -