CaMKIIβ knockdown decreases store-operated calcium entry in hippocampal dendritic spines

Nikita Zernov; Ilya Bezprozvanny; Elena Popugaeva

doi:10.1016/j.ibneur.2022.01.001

CaMKIIβ knockdown decreases store-operated calcium entry in hippocampal dendritic spines

Nikita Zernov, Ilya Bezprozvanny, Elena Popugaeva

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Calcium/calmodulin-dependent protein kinase II (CaMKII) and neuronal store-operated calcium entry (nSOCE) have been implicated in the development of Alzheimer's disease (AD). nSOCE is involved in regulation of dendritic spine shape, particularly in stability of mushroom spines that play role in formation of strong synapses. CaMKII is involved in regulation of induction of long-term potentiation, that is needed for shaping of memory. In the present study, we demonstrated that inhibition of kinase activity of CaMKII by KN-62 decreases nSOCE amplitude in soma of primary hippocampal neurons. We have shown that knockdown of CaMKIIβ leads to the downregulation of nSOCE in dendritic spines. In agreement with previously published data, we have also observed that CaMKIIβ knockdown causes mushroom spine loss in primary hippocampal culture. The effect of CaMKIIβ knockdown on the nSOCE may be associated with a decrease of dendritic spine head size.

Original language	English (US)
Pages (from-to)	90-97
Number of pages	8
Journal	IBRO Neuroscience Reports
Volume	12
DOIs	https://doi.org/10.1016/j.ibneur.2022.01.001
State	Published - Jun 2022

Keywords

Calcium imaging
Calcium/calmodulin-dependent protein kinase II
Store-operated calcium entry

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.ibneur.2022.01.001

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title = "CaMKIIβ knockdown decreases store-operated calcium entry in hippocampal dendritic spines",

abstract = "Calcium/calmodulin-dependent protein kinase II (CaMKII) and neuronal store-operated calcium entry (nSOCE) have been implicated in the development of Alzheimer's disease (AD). nSOCE is involved in regulation of dendritic spine shape, particularly in stability of mushroom spines that play role in formation of strong synapses. CaMKII is involved in regulation of induction of long-term potentiation, that is needed for shaping of memory. In the present study, we demonstrated that inhibition of kinase activity of CaMKII by KN-62 decreases nSOCE amplitude in soma of primary hippocampal neurons. We have shown that knockdown of CaMKIIβ leads to the downregulation of nSOCE in dendritic spines. In agreement with previously published data, we have also observed that CaMKIIβ knockdown causes mushroom spine loss in primary hippocampal culture. The effect of CaMKIIβ knockdown on the nSOCE may be associated with a decrease of dendritic spine head size.",

keywords = "Calcium imaging, Calcium/calmodulin-dependent protein kinase II, Store-operated calcium entry",

author = "Nikita Zernov and Ilya Bezprozvanny and Elena Popugaeva",

note = "Funding Information: We thank Dr. A. Bolshakova for administrative support. Dr. Ilya Bezprozvanny is a holder of the Carl J. and Hortense M. Thomsen Chair in Alzheimer{\textquoteright}s Disease Research. This work was supported by Russian Science Foundation Grant No. 18–74-00027 (results depicted on Figs. 1 and 2 ) and by Russian Science Foundation Grant No. 20–75-10026 (results depicted on Figs. 3−5 ). Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

month = jun,

doi = "10.1016/j.ibneur.2022.01.001",

language = "English (US)",

volume = "12",

pages = "90--97",

journal = "IBRO Neuroscience Reports",

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T1 - CaMKIIβ knockdown decreases store-operated calcium entry in hippocampal dendritic spines

AU - Zernov, Nikita

AU - Bezprozvanny, Ilya

AU - Popugaeva, Elena

N1 - Funding Information: We thank Dr. A. Bolshakova for administrative support. Dr. Ilya Bezprozvanny is a holder of the Carl J. and Hortense M. Thomsen Chair in Alzheimer’s Disease Research. This work was supported by Russian Science Foundation Grant No. 18–74-00027 (results depicted on Figs. 1 and 2 ) and by Russian Science Foundation Grant No. 20–75-10026 (results depicted on Figs. 3−5 ). Publisher Copyright: © 2022 The Authors

PY - 2022/6

Y1 - 2022/6

N2 - Calcium/calmodulin-dependent protein kinase II (CaMKII) and neuronal store-operated calcium entry (nSOCE) have been implicated in the development of Alzheimer's disease (AD). nSOCE is involved in regulation of dendritic spine shape, particularly in stability of mushroom spines that play role in formation of strong synapses. CaMKII is involved in regulation of induction of long-term potentiation, that is needed for shaping of memory. In the present study, we demonstrated that inhibition of kinase activity of CaMKII by KN-62 decreases nSOCE amplitude in soma of primary hippocampal neurons. We have shown that knockdown of CaMKIIβ leads to the downregulation of nSOCE in dendritic spines. In agreement with previously published data, we have also observed that CaMKIIβ knockdown causes mushroom spine loss in primary hippocampal culture. The effect of CaMKIIβ knockdown on the nSOCE may be associated with a decrease of dendritic spine head size.

AB - Calcium/calmodulin-dependent protein kinase II (CaMKII) and neuronal store-operated calcium entry (nSOCE) have been implicated in the development of Alzheimer's disease (AD). nSOCE is involved in regulation of dendritic spine shape, particularly in stability of mushroom spines that play role in formation of strong synapses. CaMKII is involved in regulation of induction of long-term potentiation, that is needed for shaping of memory. In the present study, we demonstrated that inhibition of kinase activity of CaMKII by KN-62 decreases nSOCE amplitude in soma of primary hippocampal neurons. We have shown that knockdown of CaMKIIβ leads to the downregulation of nSOCE in dendritic spines. In agreement with previously published data, we have also observed that CaMKIIβ knockdown causes mushroom spine loss in primary hippocampal culture. The effect of CaMKIIβ knockdown on the nSOCE may be associated with a decrease of dendritic spine head size.

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KW - Calcium/calmodulin-dependent protein kinase II

KW - Store-operated calcium entry

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CaMKIIβ knockdown decreases store-operated calcium entry in hippocampal dendritic spines

Abstract

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