cAMP-regulated chloride currents in CHO cells

Allen W. Mangel; Srisaila Basavappa; John R. Raymond; J. Gregory Fitz

doi:10.1016/S0006-291X(05)80800-9

cAMP-regulated chloride currents in CHO cells

Allen W. Mangel, Srisaila Basavappa, John R. Raymond, J. Gregory Fitz

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We examined whether elevations in cAMP levels increase membrane chloride permeability in native CHO cells by measuring whole cell chloride currents and efflux of ¹²⁵I and ³⁶Cl. With 20 μM forskolin, no significant effect was seen on whole cell currents. However, 100 μM forskolin increased both whole cell chloride currents and the rate of ¹²⁵I and ³⁶Cl efflux. Forskolin-activated currents showed a linear current-voltage relationship in solutions with symmetrical chloride concentrations and reversal potential changed in the direction anticipated for a chloride-selective current when chloride was replaced with gluconate. These results indicate that native CHO cells exhibit cAMP-regulated chloride conductance pathways which become apparent only after large elevations in intracellular cAMP levels.

Original language	English (US)
Pages (from-to)	251-255
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	186
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(05)80800-9
State	Published - Jul 15 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(05)80800-9

Cite this

@article{a348e947c8bb4343b63ac4358f4bc4f5,

title = "cAMP-regulated chloride currents in CHO cells",

abstract = "We examined whether elevations in cAMP levels increase membrane chloride permeability in native CHO cells by measuring whole cell chloride currents and efflux of 125I and 36Cl. With 20 μM forskolin, no significant effect was seen on whole cell currents. However, 100 μM forskolin increased both whole cell chloride currents and the rate of 125I and 36Cl efflux. Forskolin-activated currents showed a linear current-voltage relationship in solutions with symmetrical chloride concentrations and reversal potential changed in the direction anticipated for a chloride-selective current when chloride was replaced with gluconate. These results indicate that native CHO cells exhibit cAMP-regulated chloride conductance pathways which become apparent only after large elevations in intracellular cAMP levels.",

author = "Mangel, {Allen W.} and Srisaila Basavappa and Raymond, {John R.} and Fitz, {J. Gregory}",

note = "Funding Information: Support from NIH DK08452 (AWM) and DK43278 (JGF), a Glaxo Institute of Digestive Health Basic ResearCh Award (AWM), AGA Searle Research Scholar Award (JGF), The American Heart Association (JRR) and Veterans Administration Funding (JP/R) is acknowledged. We acknowledge technical assistance of Georgiann Collinsworth in performance of cAMP assays and maintanence of cell culture.",

year = "1992",

month = jul,

day = "15",

doi = "10.1016/S0006-291X(05)80800-9",

language = "English (US)",

volume = "186",

pages = "251--255",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - cAMP-regulated chloride currents in CHO cells

AU - Mangel, Allen W.

AU - Basavappa, Srisaila

AU - Raymond, John R.

AU - Fitz, J. Gregory

N1 - Funding Information: Support from NIH DK08452 (AWM) and DK43278 (JGF), a Glaxo Institute of Digestive Health Basic ResearCh Award (AWM), AGA Searle Research Scholar Award (JGF), The American Heart Association (JRR) and Veterans Administration Funding (JP/R) is acknowledged. We acknowledge technical assistance of Georgiann Collinsworth in performance of cAMP assays and maintanence of cell culture.

PY - 1992/7/15

Y1 - 1992/7/15

N2 - We examined whether elevations in cAMP levels increase membrane chloride permeability in native CHO cells by measuring whole cell chloride currents and efflux of 125I and 36Cl. With 20 μM forskolin, no significant effect was seen on whole cell currents. However, 100 μM forskolin increased both whole cell chloride currents and the rate of 125I and 36Cl efflux. Forskolin-activated currents showed a linear current-voltage relationship in solutions with symmetrical chloride concentrations and reversal potential changed in the direction anticipated for a chloride-selective current when chloride was replaced with gluconate. These results indicate that native CHO cells exhibit cAMP-regulated chloride conductance pathways which become apparent only after large elevations in intracellular cAMP levels.

AB - We examined whether elevations in cAMP levels increase membrane chloride permeability in native CHO cells by measuring whole cell chloride currents and efflux of 125I and 36Cl. With 20 μM forskolin, no significant effect was seen on whole cell currents. However, 100 μM forskolin increased both whole cell chloride currents and the rate of 125I and 36Cl efflux. Forskolin-activated currents showed a linear current-voltage relationship in solutions with symmetrical chloride concentrations and reversal potential changed in the direction anticipated for a chloride-selective current when chloride was replaced with gluconate. These results indicate that native CHO cells exhibit cAMP-regulated chloride conductance pathways which become apparent only after large elevations in intracellular cAMP levels.

UR - http://www.scopus.com/inward/record.url?scp=0026757531&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026757531&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(05)80800-9

DO - 10.1016/S0006-291X(05)80800-9

M3 - Article

C2 - 1321606

AN - SCOPUS:0026757531

SN - 0006-291X

VL - 186

SP - 251

EP - 255

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

cAMP-regulated chloride currents in CHO cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this