Canonical transient receptor potential channels promote cardiomyocyte hypertrophy through activation of calcineurin signaling

Erik W. Bush, David B. Hood, Philip J. Papst, Joseph A. Chapo, Wayne Minobe, Michael R. Bristow, Eric N. Olson, Timothy A. McKinsey

Research output: Contribution to journalArticle

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Abstract

The calcium/calmodulin-dependent phosphatase calcineurin plays a central role in the control of cardiomyocyte hypertrophy in response to pathological stimuli. Although calcineurin is present at high levels in normal heart, its activity appears to be unaffected by calcium during the course of a cardiac cycle. The mechanism(s) whereby calcineurin is selectively activated by calcium under pathological conditions has remained unclear. Here, we demonstrate that diverse signals for cardiac hypertrophy stimulate expression of canonical transient receptor potential (TRPC) channels. TRPC consists of a family of seven membrane-spanning nonselective cation channels that have been implicated in the nonvoltage-gated influx of calcium in response to G protein-coupled receptor signaling, receptor tyrosine kinase signaling, and depletion of internal calcium stores. TRPC3 expression is up-regulated in multiple rodent models of pathological cardiac hypertrophy, whereas TRPC5 expression is induced in failing human heart. We demonstrate that TRPC promotes cardiomyocyte hypertrophy through activation of calcineurin and its downstream effector, the nuclear factor of activated T cells transcription factor. These results define a novel role for TRPC channels in the control of cardiac growth, and suggest that a TRPC-derived pool of calcium contributes to selective activation of calcineurin in diseased heart.

Original languageEnglish (US)
Pages (from-to)33487-33496
Number of pages10
JournalJournal of Biological Chemistry
Volume281
Issue number44
DOIs
StatePublished - Nov 3 2006

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Transient Receptor Potential Channels
Calcineurin
Cardiac Myocytes
Hypertrophy
Chemical activation
Calcium
Cardiomegaly
NFATC Transcription Factors
TCF Transcription Factors
Receptor Protein-Tyrosine Kinases
Calmodulin
G-Protein-Coupled Receptors
Cations
Rodentia
Heart Diseases
Transcription Factors
Membranes
Growth

ASJC Scopus subject areas

  • Biochemistry

Cite this

Bush, E. W., Hood, D. B., Papst, P. J., Chapo, J. A., Minobe, W., Bristow, M. R., ... McKinsey, T. A. (2006). Canonical transient receptor potential channels promote cardiomyocyte hypertrophy through activation of calcineurin signaling. Journal of Biological Chemistry, 281(44), 33487-33496. https://doi.org/10.1074/jbc.M605536200

Canonical transient receptor potential channels promote cardiomyocyte hypertrophy through activation of calcineurin signaling. / Bush, Erik W.; Hood, David B.; Papst, Philip J.; Chapo, Joseph A.; Minobe, Wayne; Bristow, Michael R.; Olson, Eric N.; McKinsey, Timothy A.

In: Journal of Biological Chemistry, Vol. 281, No. 44, 03.11.2006, p. 33487-33496.

Research output: Contribution to journalArticle

Bush, Erik W. ; Hood, David B. ; Papst, Philip J. ; Chapo, Joseph A. ; Minobe, Wayne ; Bristow, Michael R. ; Olson, Eric N. ; McKinsey, Timothy A. / Canonical transient receptor potential channels promote cardiomyocyte hypertrophy through activation of calcineurin signaling. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 44. pp. 33487-33496.
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