Carbon flux through citric acid cycle pathways in perfused heart by 13C NMR spectroscopy

Craig R. Malloy, A. Dean Sherry, F. Mark H Jeffrey

Research output: Contribution to journalArticle

109 Scopus citations

Abstract

Mathematical models of the TCA cycle derived previously for 14C tracer studies have been extended to 13C NMR to measure the 13C fractional enrichment of [2-13C]acetyl-CoA entering the cycle and the relative activities of the oxidative versus anaplerotic pathways. The analysis is based upon the steady-state enrichment of 13C into the glutamate carbons. Hearts perfused with [2-13C]acetate show low but significant activity of the anaplerotic pathways. Activation of two different anaplerotic pathways is demonstrated by addition of unlabeled propionate or pyruvate to hearts perfused with [2-13C]acetate. In each case, the amount of [2-13C]acetate being oxidized and the relative carbon flux through anaplerotic versus oxidative pathways are evaluated.

Original languageEnglish (US)
Pages (from-to)58-62
Number of pages5
JournalFEBS Letters
Volume212
Issue number1
DOIs
StatePublished - Feb 9 1987

Keywords

  • (Perfused heart)
  • -C NMR
  • Glutamate isotopomer

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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