Carbon flux through citric acid cycle pathways in perfused heart by 13C NMR spectroscopy

Craig R. Malloy, A. Dean Sherry, F. Mark H Jeffrey

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Mathematical models of the TCA cycle derived previously for 14C tracer studies have been extended to 13C NMR to measure the 13C fractional enrichment of [2-13C]acetyl-CoA entering the cycle and the relative activities of the oxidative versus anaplerotic pathways. The analysis is based upon the steady-state enrichment of 13C into the glutamate carbons. Hearts perfused with [2-13C]acetate show low but significant activity of the anaplerotic pathways. Activation of two different anaplerotic pathways is demonstrated by addition of unlabeled propionate or pyruvate to hearts perfused with [2-13C]acetate. In each case, the amount of [2-13C]acetate being oxidized and the relative carbon flux through anaplerotic versus oxidative pathways are evaluated.

Original languageEnglish (US)
Pages (from-to)58-62
Number of pages5
JournalFEBS Letters
Volume212
Issue number1
DOIs
StatePublished - Feb 9 1987

Keywords

  • (Perfused heart)
  • -C NMR
  • Glutamate isotopomer

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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