CARD9 mediates dectin-2-induced IκBα kinase ubiquitination leading to activation of NF-κB in response to stimulation by the hyphal form of Candida albicans

Liangkuan Bi, Sara Gojestani, Weihui Wu, Yen Michael S Hsu, Jiayuan Zhu, Kiyoshi Ariizumi, Xin Lin

Research output: Contribution to journalArticle

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Abstract

The scaffold protein CARD9 plays an essential role in antifungus immunity and is implicated in mediating Dectin-1/Syk-induced NF-κB activation in response to Candida albicans infection. However, the molecular mechanism by which CARD9 mediates C. albicans-induced NF-κB activation is not fully characterized. Here we demonstrate that CARD9 is involved in mediating NF-κB activation induced by the hyphal form of C. albicans hyphae (Hyphae) but not by its heat-inactivated unicellular form. Our data show that inhibiting Dectin-2 expression selectively blocked Hyphae-induced NF-κB, whereas inhibiting Dectin-1 mainly suppressed zymosan-induced NF-κB, indicating that Hyphae-induced NF-κB activation is mainly through Dectin-2 and not Dectin-1. Consistently, we find that the hyphae stimulation induces CARD9 association with Bcl10, an adaptor protein that functions downstream of CARD9 and is also involved in C. albicans-induced NF-κB activation. This association is dependent on Dectin-2 but not Dectin-1 following the hyphae stimulation. Finally, we find that although both CARD9 and Syk are required for Hyphae-induced NF-κB activation, they regulate different signaling events in which CARD9 mediates IκBα kinase ubiquitination, whereas Syk regulates IκBα kinase phosphorylation. Together, our data demonstrated that CARD9 is selectively involved in Dectin-2-induced NF-κB activation in response to C. albicans hyphae challenging.

Original languageEnglish (US)
Pages (from-to)25969-25977
Number of pages9
JournalJournal of Biological Chemistry
Volume285
Issue number34
DOIs
StatePublished - Aug 20 2010

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Forms (concrete)
Hyphae
Candida
Ubiquitination
Candida albicans
Phosphotransferases
Chemical activation
Association reactions
Phosphorylation
Zymosan
mouse dectin-2
Scaffolds
Proteins
Immunity
Hot Temperature
dectin 1
Infection

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

CARD9 mediates dectin-2-induced IκBα kinase ubiquitination leading to activation of NF-κB in response to stimulation by the hyphal form of Candida albicans. / Bi, Liangkuan; Gojestani, Sara; Wu, Weihui; Hsu, Yen Michael S; Zhu, Jiayuan; Ariizumi, Kiyoshi; Lin, Xin.

In: Journal of Biological Chemistry, Vol. 285, No. 34, 20.08.2010, p. 25969-25977.

Research output: Contribution to journalArticle

Bi, Liangkuan ; Gojestani, Sara ; Wu, Weihui ; Hsu, Yen Michael S ; Zhu, Jiayuan ; Ariizumi, Kiyoshi ; Lin, Xin. / CARD9 mediates dectin-2-induced IκBα kinase ubiquitination leading to activation of NF-κB in response to stimulation by the hyphal form of Candida albicans. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 34. pp. 25969-25977.
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abstract = "The scaffold protein CARD9 plays an essential role in antifungus immunity and is implicated in mediating Dectin-1/Syk-induced NF-κB activation in response to Candida albicans infection. However, the molecular mechanism by which CARD9 mediates C. albicans-induced NF-κB activation is not fully characterized. Here we demonstrate that CARD9 is involved in mediating NF-κB activation induced by the hyphal form of C. albicans hyphae (Hyphae) but not by its heat-inactivated unicellular form. Our data show that inhibiting Dectin-2 expression selectively blocked Hyphae-induced NF-κB, whereas inhibiting Dectin-1 mainly suppressed zymosan-induced NF-κB, indicating that Hyphae-induced NF-κB activation is mainly through Dectin-2 and not Dectin-1. Consistently, we find that the hyphae stimulation induces CARD9 association with Bcl10, an adaptor protein that functions downstream of CARD9 and is also involved in C. albicans-induced NF-κB activation. This association is dependent on Dectin-2 but not Dectin-1 following the hyphae stimulation. Finally, we find that although both CARD9 and Syk are required for Hyphae-induced NF-κB activation, they regulate different signaling events in which CARD9 mediates IκBα kinase ubiquitination, whereas Syk regulates IκBα kinase phosphorylation. Together, our data demonstrated that CARD9 is selectively involved in Dectin-2-induced NF-κB activation in response to C. albicans hyphae challenging.",
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AU - Wu, Weihui

AU - Hsu, Yen Michael S

AU - Zhu, Jiayuan

AU - Ariizumi, Kiyoshi

AU - Lin, Xin

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