Cardiomyocyte-specific deletion of endothelin receptor A rescues aging-associated cardiac hypertrophy and contractile dysfunction

Role of autophagy

Asli F. Ceylan-Isik, Maolong Dong, Yingmei Zhang, Feng Dong, Subat Turdi, Sreejayan Nair, Masashi Yanagisawa, Jun Ren

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Cardiac aging is manifested as cardiac remodeling and contractile dysfunction although precise mechanisms remain elusive. This study was designed to examine the role of endothelin-1 (ET-1) in aging-associated myocardial morphological and contractile defects. Echocardiographic and cardiomyocyte contractile properties were evaluated in young (5-6 months) and old (26-28 months) C57BL/6 wild-type and cardiomyocytespecific ETA receptor knockout (ETAKO) mice. Cardiac ROS production and histology were examined. Our data revealed that ETAKO mice displayed an improved survival. Aging increased plasma levels of ET-1 and Ang II, compromised cardiac function (fractional shortening, cardiomyocyte peak shortening, maximal velocity of shortening/relengthening and prolonged relengthening) and intracellular Ca 2+ handling (reduced intracellular Ca2+ release and decay), the effects of which with the exception of ET-1 and Ang II levels was improved by ETAKO. Histological examination displayed cardiomyocyte hypertrophy and interstitial fibrosis associated with cardiac remodeling in aged C57 mice, which were alleviated in ETAKO mice. Aging promoted ROS generation, protein damage, ER stress, upregulated GATA4, ANP, NFATc3 and the autophagosome cargo protein p62, downregulated intracellular Ca2+ regulatory proteins SERCA2a and phospholamban as well as the autophagic markers Beclin- 1, Atg7, Atg5 and LC3BII, which were ablated by ETAKO. ET-1 triggered a decrease in autophagy and increased hypertrophic markers in vitro, the effect of which were reversed by the ETA receptor antagonist BQ123 and the autophagy inducer rapamycin. Antagonism of ETA, but not ETB receptor, rescued cardiac aging, which was negated by autophagy inhibition. Taken together, our data suggest that cardiac ETA receptor ablation protects against aging-associated myocardial remodeling and contractile dysfunction possibly through autophagy regulation.

Original languageEnglish (US)
Article number335
JournalBasic Research in Cardiology
Volume108
Issue number2
DOIs
StatePublished - Jan 1 2013

Fingerprint

Endothelin A Receptors
Autophagy
Cardiomegaly
Endothelin-1
Cardiac Myocytes
Knockout Mice
Proteins
Atrial Natriuretic Factor
Sirolimus
Hypertrophy
Histology
Fibrosis
Down-Regulation

Keywords

  • Autophagy
  • Cardiomyocyte
  • Contraction
  • ET receptor
  • Morphology
  • Myocardial

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)
  • Physiology

Cite this

Cardiomyocyte-specific deletion of endothelin receptor A rescues aging-associated cardiac hypertrophy and contractile dysfunction : Role of autophagy. / Ceylan-Isik, Asli F.; Dong, Maolong; Zhang, Yingmei; Dong, Feng; Turdi, Subat; Nair, Sreejayan; Yanagisawa, Masashi; Ren, Jun.

In: Basic Research in Cardiology, Vol. 108, No. 2, 335, 01.01.2013.

Research output: Contribution to journalArticle

Ceylan-Isik, Asli F. ; Dong, Maolong ; Zhang, Yingmei ; Dong, Feng ; Turdi, Subat ; Nair, Sreejayan ; Yanagisawa, Masashi ; Ren, Jun. / Cardiomyocyte-specific deletion of endothelin receptor A rescues aging-associated cardiac hypertrophy and contractile dysfunction : Role of autophagy. In: Basic Research in Cardiology. 2013 ; Vol. 108, No. 2.
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AB - Cardiac aging is manifested as cardiac remodeling and contractile dysfunction although precise mechanisms remain elusive. This study was designed to examine the role of endothelin-1 (ET-1) in aging-associated myocardial morphological and contractile defects. Echocardiographic and cardiomyocyte contractile properties were evaluated in young (5-6 months) and old (26-28 months) C57BL/6 wild-type and cardiomyocytespecific ETA receptor knockout (ETAKO) mice. Cardiac ROS production and histology were examined. Our data revealed that ETAKO mice displayed an improved survival. Aging increased plasma levels of ET-1 and Ang II, compromised cardiac function (fractional shortening, cardiomyocyte peak shortening, maximal velocity of shortening/relengthening and prolonged relengthening) and intracellular Ca 2+ handling (reduced intracellular Ca2+ release and decay), the effects of which with the exception of ET-1 and Ang II levels was improved by ETAKO. Histological examination displayed cardiomyocyte hypertrophy and interstitial fibrosis associated with cardiac remodeling in aged C57 mice, which were alleviated in ETAKO mice. Aging promoted ROS generation, protein damage, ER stress, upregulated GATA4, ANP, NFATc3 and the autophagosome cargo protein p62, downregulated intracellular Ca2+ regulatory proteins SERCA2a and phospholamban as well as the autophagic markers Beclin- 1, Atg7, Atg5 and LC3BII, which were ablated by ETAKO. ET-1 triggered a decrease in autophagy and increased hypertrophic markers in vitro, the effect of which were reversed by the ETA receptor antagonist BQ123 and the autophagy inducer rapamycin. Antagonism of ETA, but not ETB receptor, rescued cardiac aging, which was negated by autophagy inhibition. Taken together, our data suggest that cardiac ETA receptor ablation protects against aging-associated myocardial remodeling and contractile dysfunction possibly through autophagy regulation.

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KW - Morphology

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