Cardiovascular dysfunction caused by cecal ligation and puncture is attenuated in CD8 knockout mice treated with anti-asialoGM1

Weike Tao, Victor T. Enoh, Cheng Y. Lin, William E. Johnston, Peng Li, Edward R. Sherwood

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The present study was designed to assess hemodynamics and myocardial function at 18 h after injury caused by cecal ligation and puncture (CLP) in CD8-knockout mice treated with anti-asialoGM1 (CD8KO/αAsGM1 mice). Arterial pressure was measured by carotid artery cannulation, and left ventricular pressure-volume measurements were obtained by use of a 1.4-Fr conductance catheter. Blood acid-base balance and indexes of hepatic, renal, and pulmonary injury were also measured. CD8KO/αAsGM1 mice exhibited higher mean arterial pressure and increased systemic vascular resistance compared with wild-type mice. Cardiac output was significantly decreased in wild-type, but not CD8KO/αAsGM1, mice compared with sham controls. Myocardial function was better preserved in CD8KO/αAsGM1 mice as indicated by less impairment of left ventricular pressure development over time, time varying maximum elastance, end-systolic pressure-volume relationship, and preload recruitable stroke work. The impairment in myocardial function was associated with induction of proinflammatory cytokine mRNAs in the hearts of wild-type mice. The hemodynamic derangements in wild-type mice were coupled with significant metabolic acidosis and elevated serum creatinine levels. Overall, this study shows that cardiovascular collapse and shock characterized by hypotension, myocardial depression, low systemic vascular resistance, and metabolic acidosis occurs after CLP in wild-type mice but is attenuated in CD8KO/αAsGM1 mice. These observations likely explain, in part, the previously observed survival advantage of CD8KO/ αAsGM1 mice following CLP.

Original languageEnglish (US)
Pages (from-to)R478-R485
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume289
Issue number2 58-2
DOIs
StatePublished - Aug 1 2005

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Keywords

  • Blood pressure
  • Cardiac output
  • Left ventricular function
  • Perfusion
  • Vascular resistance

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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