Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization

Juying Li, William M. Brieher, M. Lucila Scimone, Shin Jung Kang, Hong Zhu, Helen Yin, Ulrich H. von Andrian, Timothy Mitchison, Junying Yuan

Research output: Contribution to journalArticle

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Abstract

Coordinated regulation of cell migration, cytokine maturation and apoptosis is critical in inflammatory responses. Caspases, a family of cysteine proteases, are known to regulate cytokine maturation and apoptosis. Here, we show that caspase-11, a mammalian pro-inflammatory caspase, regulates cell migration during inflammation. Caspase-11-deficient lymphocytes exhibit a cell-autonomous migration defect in vitro and in vivo. We demonstrate that caspase-11 interacts physically and functionally with actin interacting protein 1 (Aip1), an activator of cofilin-mediated actin depolymerization. The caspase-recruitment domain (CARD) of caspase-11 interacts with the carboxy-terminal WD40 propeller domain of Aip1 to promote cofilin-mediated actin depolymerization. Cells with Aip1 or caspase-11 deficiency exhibit defects in actin dynamics. Using in vitro actin depolymerization assays, we found that caspase-11 and Aip1 work cooperatively to promote cofilin-mediated actin depolymerization. These data demonstrate a novel cell autonomous caspase-mediated mechanism that regulates actin dynamics and mammalian cell migration distinct from the receptor mediated Rho-Rac-Cdc42 pathway.

Original languageEnglish (US)
Pages (from-to)276-286
Number of pages11
JournalNature Cell Biology
Volume9
Issue number3
DOIs
StatePublished - Mar 2007

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Actin Depolymerizing Factors
Caspases
Cell Movement
Actins
Apoptosis
Cytokines
actin interacting protein 1
Cysteine Proteases
Lymphocytes
Inflammation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Li, J., Brieher, W. M., Scimone, M. L., Kang, S. J., Zhu, H., Yin, H., ... Yuan, J. (2007). Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization. Nature Cell Biology, 9(3), 276-286. https://doi.org/10.1038/ncb1541

Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization. / Li, Juying; Brieher, William M.; Scimone, M. Lucila; Kang, Shin Jung; Zhu, Hong; Yin, Helen; von Andrian, Ulrich H.; Mitchison, Timothy; Yuan, Junying.

In: Nature Cell Biology, Vol. 9, No. 3, 03.2007, p. 276-286.

Research output: Contribution to journalArticle

Li, J, Brieher, WM, Scimone, ML, Kang, SJ, Zhu, H, Yin, H, von Andrian, UH, Mitchison, T & Yuan, J 2007, 'Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization', Nature Cell Biology, vol. 9, no. 3, pp. 276-286. https://doi.org/10.1038/ncb1541
Li, Juying ; Brieher, William M. ; Scimone, M. Lucila ; Kang, Shin Jung ; Zhu, Hong ; Yin, Helen ; von Andrian, Ulrich H. ; Mitchison, Timothy ; Yuan, Junying. / Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization. In: Nature Cell Biology. 2007 ; Vol. 9, No. 3. pp. 276-286.
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