CASTing for multicomponent DNA-binding complexes

Woodring E. Wright, Walter D. Funk

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Degenerate oligonucleotides and polymerase chain reaction-based reiterative selection techniques have been used to define the consensus binding sites for an increasing number of transcription factors. The use of crude nuclear extracts rather than purified proteins permits multicomponent complexes to form, and allows the technique to generate information about the combinatorial interactions involved in gene regulation.

Original languageEnglish (US)
Pages (from-to)77-80
Number of pages4
JournalTrends in biochemical sciences
Volume18
Issue number3
DOIs
StatePublished - Mar 1993

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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