CATALYTIC AND SPECTROSCOPIC PROPERTIES OF THE LANTHANIDE ION ALKALINE PHOSPHATASE COMPLEX.

G. L. Cottam, A. D. Sherry, K. M. Valentine

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

The addition of lanthanide ions to catalytically inactive apo alkaline phosphatase results in less than 5 percent of the native catalytic activity. Attempts to distinquish whether this catalytic activity results for a lanthanide ion-alkaline phosphatase complex or contamination with zinc ion have thus for been unsuccessful. In an attempt to examine the role of the metal ions in the enzymic reaction catalyzed by alkaline phosphatase attempts have been made to demonstrate whether substrates enter the primary coordination sphere of the bound metal. Addition of glucose-6-phosphate, phosphoenolypyruvate or inorganic phosphate result in a quenching of the luminescence of the terbium-alkaline phosphatase complex. This decrease in luminescence is a result of a change in the environment around the bound terbium ion either by direct binding of substrate to the terbium or a substrate induced conformational change.

Original languageEnglish (US)
Title of host publicationUnknown Host Publication Title
Place of PublicationOak Ridge, Tenn
PublisherAEC
Pages204-212
Number of pages9
StatePublished - 1800
EventRare Earth Res Conf, 11th, Proc - Traverse City, MI, USA
Duration: Oct 7 1974Oct 10 1974

Other

OtherRare Earth Res Conf, 11th, Proc
CityTraverse City, MI, USA
Period10/7/7410/10/74

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ASJC Scopus subject areas

  • Engineering(all)

Cite this

Cottam, G. L., Sherry, A. D., & Valentine, K. M. (1800). CATALYTIC AND SPECTROSCOPIC PROPERTIES OF THE LANTHANIDE ION ALKALINE PHOSPHATASE COMPLEX. In Unknown Host Publication Title (pp. 204-212). AEC.