Kataliticheskie svoǐstva vnekletochnykh fenoloksidaz vysshego bazidiomitseta Pleurotus ostreatus (Fr.) Kumm.

Translated title of the contribution: Catalytic properties of extracellular phenol oxidases from the higher basidiomycete Pleurotus ostreatus (Fr.) Kumm

I. A. Butovich, V. D. Semichaevskiǐ

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Phenol oxidase preparations possessing high stability in the reaction of phenols oxidation are isolated from the culture filtrates of four strains of higher basidiomycete Pleurotus ostreatus (F r.) Kumm. The preparations are able to oxidize mono- and diphenols with different substituents in o-, m- and p-positions to phenol hydroxyls as well as o- and p-phenylene diamines. A change in the orientation of substituents in the substrate molecule is followed by a change in the mechanism of its oxidation. The enzyme activity is determined by the type of substituents in the phenol molecule: electron-withdrawing groups impede and electron-releasing ones facilitate the substrate oxidation. The Hammett equation is shown to be applicable for description of monophenol oxidation. An explanation of high values of positive deviations from it in the case of halogen-substituted phenols is suggested. A conclusion is drawn that Km of enzymes for oxygen determined from the full kinetic curves of O2 uptake with phenol redundancy are kinetic constants depending on the substrate structure.

Original languageUndefined
Pages (from-to)18-26
Number of pages9
JournalUkrainskii biokhimicheskii zhurnal
Volume58
Issue number4
StatePublished - Jul 1986

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Pleurotus
Basidiomycota
Monophenol Monooxygenase
Phenol
Phenols
Oxidation
Electrons
Halogens
Diamines
Substrates
Enzymes
Hydroxyl Radical
Molecules
Kinetics
Enzyme activity
Oxygen
Redundancy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Kataliticheskie svoǐstva vnekletochnykh fenoloksidaz vysshego bazidiomitseta Pleurotus ostreatus (Fr.) Kumm. / Butovich, I. A.; Semichaevskiǐ, V. D.

In: Ukrainskii biokhimicheskii zhurnal, Vol. 58, No. 4, 07.1986, p. 18-26.

Research output: Contribution to journalArticle

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N2 - Phenol oxidase preparations possessing high stability in the reaction of phenols oxidation are isolated from the culture filtrates of four strains of higher basidiomycete Pleurotus ostreatus (F r.) Kumm. The preparations are able to oxidize mono- and diphenols with different substituents in o-, m- and p-positions to phenol hydroxyls as well as o- and p-phenylene diamines. A change in the orientation of substituents in the substrate molecule is followed by a change in the mechanism of its oxidation. The enzyme activity is determined by the type of substituents in the phenol molecule: electron-withdrawing groups impede and electron-releasing ones facilitate the substrate oxidation. The Hammett equation is shown to be applicable for description of monophenol oxidation. An explanation of high values of positive deviations from it in the case of halogen-substituted phenols is suggested. A conclusion is drawn that Km of enzymes for oxygen determined from the full kinetic curves of O2 uptake with phenol redundancy are kinetic constants depending on the substrate structure.

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