Abstract
Caveolin-1 (Cav-1) was originally identified as a structural protein of caveolae, which is a plasma membrane domain that regulates a variety of signaling pathways involved in cell growth and migration. Here, we show that expression of Cav-1 in the Cav-1-deficient human prostate cancer cell line LNCaP both stimulates cell proliferation and promotes tumor growth in nude mice. Unexpectedly, Cav-1 expressing LNCaP (LNCaPCav-1) cells injected into one side of a nude mouse promoted tumor growth of Cav-1 negative LNCaP cells injected on the contralateral side of the same animal. The LNCaP tumors were positive for Cav-1, however, this signal was not caused by migrated LNCaP Cav-1 cells, but we show that this Cav-1 was secreted by the LNCaPCav-1 tumors. We demonstrate that conditioned media from LNCaPCav-1 cells contained Cav-1 that was associated with a lipoprotein particle ranging in size from 15 to 30 nm and a density similar to high density lipoprotein particle. These results suggest that LNCaP Cav-1 cells secreting Cav-1 particle produce an endocrine factor that stimulates tumor growth.
Original language | English (US) |
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Pages (from-to) | 520-525 |
Number of pages | 6 |
Journal | International Journal of Cancer |
Volume | 122 |
Issue number | 3 |
DOIs | |
State | Published - Feb 1 2008 |
Keywords
- Caveolin-1
- LNCaP
- Lipoprotein particle
- Prostate cancer
- Secretion
ASJC Scopus subject areas
- Oncology
- Cancer Research