CBA/N X-linked B-cell defect prevents NZB B-cell hyperactivity in F 1 mice

J. D. Taurog, H. M. Moutsopoulos, Y. J. Rosenberg, T. M. Chused, A. D. Steinberg

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Abstract

NZB mice and their F 1 hybrids produce excessive polyclonal IgM and autoantibodies of both IgM and IgG classes. CBA/N mice and CBA/N-mothered F 1 males fail to make antibody to many T-independent antigens and have low levels of serum IgM; further, these mice lack a population of splenic B cells characterized by a low-to-intermediate density of surface IgM. We have studied male CBA/N, NZB, CBA/N x NZB, NZB x CBA/N, and CBA/J mice; female CBA/N x NZB mice; and males of several control crosses of NZB and CBA/N mice. We have found that the CBA/N X-linked defect of T-independent immune response is completely expressed in CBA/N x NZB mice. In marked contrast to NZB mice and to NZB F 1 hybrids bearing at least one normal X chromosome, the CBA/N x NZB males failed to respond to two T-independent antigens, had small numbers of splenic IgM-producing cells, barely detectable splenic IgM production, and splenic B-cell surface-Ig patterns resembling those of CBA/N mice. These data suggest that the NZB B-cell abnormality resulting in excessive IgM production occurs almost exclusively in that population of B cells affected by the CBA/N X-chromosome-linked defect. Preliminary studies suggest that the CBA/N X chromosome retards the spontaneous development of anti-erythrocyte autoantibodies in CBA/N x NZB males. Castration, known to accelerate autoimmune disease in certain NZB F 1 males, appears to have no influence on the immune functions examined in this study.

Original languageEnglish (US)
Pages (from-to)31-43
Number of pages13
JournalJournal of Experimental Medicine
Volume150
Issue number1
DOIs
StatePublished - 1979

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Immunoglobulin M
Inbred NZB Mouse
B-Lymphocytes
Inbred CBA Mouse
X Chromosome
T Independent Antigens
Autoantibodies
Castration
Population
Autoimmune Diseases
Immunoglobulin G
Erythrocytes
Antibodies
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

CBA/N X-linked B-cell defect prevents NZB B-cell hyperactivity in F 1 mice. / Taurog, J. D.; Moutsopoulos, H. M.; Rosenberg, Y. J.; Chused, T. M.; Steinberg, A. D.

In: Journal of Experimental Medicine, Vol. 150, No. 1, 1979, p. 31-43.

Research output: Contribution to journalArticle

Taurog, JD, Moutsopoulos, HM, Rosenberg, YJ, Chused, TM & Steinberg, AD 1979, 'CBA/N X-linked B-cell defect prevents NZB B-cell hyperactivity in F 1 mice', Journal of Experimental Medicine, vol. 150, no. 1, pp. 31-43. https://doi.org/10.1084/jem.150.1.31
Taurog, J. D. ; Moutsopoulos, H. M. ; Rosenberg, Y. J. ; Chused, T. M. ; Steinberg, A. D. / CBA/N X-linked B-cell defect prevents NZB B-cell hyperactivity in F 1 mice. In: Journal of Experimental Medicine. 1979 ; Vol. 150, No. 1. pp. 31-43.
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