CD11b and intercellular adhesion molecule-1 are involved in pulmonary neutrophil recruitment in lipopolysaccharide-induced airway disease

Jessica G. Moreland, Robert M. Fuhrman, Jonathan A. Pruessner, David A. Schwartz

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

To better define the roles of CD11b, CD11a, and one of their endothelial cell receptors, intercellular adhesion molecule-1 (ICAM-1), in the lower respiratory tract inflammatory response to inhaled lipopolysaccharide (LPS), we evaluated the physiologic and biologic response to inhaled LPS in mice receiving anti-CD11b antibody, anti-CD11a antibody, and anti-ICAM-1 antibody. Mice receiving anti-CD11b antibody had a dramatic reduction in pulmonary neutrophil recruitment compared with control mice (18,300 versus 143,000 cells/ml, and neutrophils 16.7% versus 77%), whereas mice receiving anti-CD11a antibody did not demonstrate a reduction in lavage cellularity. Mice receiving anti-ICAM-1 antibody also demonstrated a dose-dependent reduction in inflammatory cell recruitment to the alveolar space. Despite the significant reduction in inflammatory cell infiltrate in mice receiving either CD11b or ICAM-1 antibodies, there was no reduction in the development of airway hyperreactivity. These findings suggest that CD11b and ICAM-1 are important mediators of LPS-induced airway inflammation, but do not appear to be critical to the development of LPS-induced airway hyperreactivity.

Original languageEnglish (US)
Pages (from-to)474-480
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume27
Issue number4
StatePublished - Oct 2002

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Neutrophil Infiltration
Intercellular Adhesion Molecule-1
Lipopolysaccharides
Lung
Antibodies
Anti-Idiotypic Antibodies
Endothelial cells
Therapeutic Irrigation
Respiratory System
Neutrophils
Endothelial Cells
Inflammation

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

CD11b and intercellular adhesion molecule-1 are involved in pulmonary neutrophil recruitment in lipopolysaccharide-induced airway disease. / Moreland, Jessica G.; Fuhrman, Robert M.; Pruessner, Jonathan A.; Schwartz, David A.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 27, No. 4, 10.2002, p. 474-480.

Research output: Contribution to journalArticle

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