CDK5RAP2 regulates centriole engagement and cohesion in mice

Jose A. Barrera, Ling Rong Kao, Robert E Hammer, Joachim Seemann, Jannon L. Fuchs, Timothy L. Megraw

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

Centriole duplication occurs once per cell cycle, ensuring that each cell contains two centrosomes, each containing a mother-daughter pair of tightly engaged centrioles at mitotic entry. Loss of the tight engagement betweenmother and daughter centrioles appears to license the next round of centriole duplication. However, the molecular mechanisms regulating this process remain largely unknown. Mutations in CDK5RAP2, which encodes a centrosomal protein, cause autosomal recessive primary microcephaly in humans. Here we show that CDK5RAP2 loss of function in mice causes centriole amplification with a preponderance of single, unpaired centrioles and increased numbers of daughter-daughter centriole pairs. These results indicate that CDK5RAP2 is required to maintain centriole engagement and cohesion, thereby restricting centriole replication. Early in mitosis, amplified centrosomes assemble multipolar spindles in CDK5RAP2 mutant cells. Moreover, both mother and daughter centrioles are amplified and the excess mother centrioles template multiple primary cilia in CDK5RAP2 mutant cells.

Original languageEnglish (US)
Pages (from-to)913-926
Number of pages14
JournalDevelopmental cell
Volume18
Issue number6
DOIs
StatePublished - Mar 16 2010

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ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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