cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9)

Takeshi K. Watanabe, Akihiko Saito, Mikio Suzuki, Tsutomu Fujiwara, Ei ichi Takahashi, Clive A. Slaughter, George N. DeMartino, Klavs B. Hendil, Chin H. Chung, Nobuyuki Tanahashi, Keiji Tanaka

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We have employed cDNA cloning to deduce the complete primary structure of a new subunit, designated p27, of the modulator trimer complex that stimulates the association of the PA700 regulator with the catalytic 20S proteasome to form the ATP-dependent active 26S proteasome. We found two distinct cDNAs encoding two highly homologous proteins except in the C- terminal region, which are termed tentatively p27-1 and p27-2. The short p27- 2 cDNA has a deletion of 65 bp near the 3'-end region of the long p27-1 cDNA, which encodes a large protein with an extended C-terminal region, designated p27-L, whereas the long p27-1 cDNA encodes a small protein named p27-S. The polypeptides of p27-L and p27-S consist of 223 and 209 amino acid residues with calculated molecular masses of 24,852 and 22,764 and isoelectric points of 6.50 and 5.28, respectively. Immunoblot analysis with anti-p27 antibody revealed that p27, together with two other ATPase components, TBP1 and p42, was associated with not only the modulator complex but also significantly with the 26S proteasome complex, suggesting that the three are common/sharing subunits in these two complexes. By the fluorescence in situ hybridization method, the p27 (PSMD9) gene was mapped to the q24.2-q24.3 band of human chromosome 12. Computer-assisted homology analysis revealed the high sequence similarities of p27-L with a possible counterpart in Caenorhabditis elegans and Saccharomyces cerevisiae whose function is yet unknown, the yeast gene that is here termed NAS2 (non-ATPase subunit 2). Disruption of NAS2 had no effect on cell viability, indicating that the subunit is not essential for proliferation of yeast cells.

Original languageEnglish (US)
Pages (from-to)241-250
Number of pages10
JournalGenomics
Volume50
Issue number2
DOIs
StatePublished - Jun 1 1998

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Organism Cloning
Complementary DNA
Yeasts
Chromosomes, Human, Pair 12
Proteins
Caenorhabditis elegans
Isoelectric Point
Human Chromosomes
Proteasome Endopeptidase Complex
Fluorescence In Situ Hybridization
Genes
Saccharomyces cerevisiae
Adenosine Triphosphatases
Anti-Idiotypic Antibodies
Cell Survival
Adenosine Triphosphate
Cell Proliferation
human PSMD9 protein
Amino Acids
Peptides

ASJC Scopus subject areas

  • Genetics

Cite this

Watanabe, T. K., Saito, A., Suzuki, M., Fujiwara, T., Takahashi, E. I., Slaughter, C. A., ... Tanaka, K. (1998). cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9). Genomics, 50(2), 241-250. https://doi.org/10.1006/geno.1998.5301

cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9). / Watanabe, Takeshi K.; Saito, Akihiko; Suzuki, Mikio; Fujiwara, Tsutomu; Takahashi, Ei ichi; Slaughter, Clive A.; DeMartino, George N.; Hendil, Klavs B.; Chung, Chin H.; Tanahashi, Nobuyuki; Tanaka, Keiji.

In: Genomics, Vol. 50, No. 2, 01.06.1998, p. 241-250.

Research output: Contribution to journalArticle

Watanabe, TK, Saito, A, Suzuki, M, Fujiwara, T, Takahashi, EI, Slaughter, CA, DeMartino, GN, Hendil, KB, Chung, CH, Tanahashi, N & Tanaka, K 1998, 'cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9)', Genomics, vol. 50, no. 2, pp. 241-250. https://doi.org/10.1006/geno.1998.5301
Watanabe TK, Saito A, Suzuki M, Fujiwara T, Takahashi EI, Slaughter CA et al. cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9). Genomics. 1998 Jun 1;50(2):241-250. https://doi.org/10.1006/geno.1998.5301
Watanabe, Takeshi K. ; Saito, Akihiko ; Suzuki, Mikio ; Fujiwara, Tsutomu ; Takahashi, Ei ichi ; Slaughter, Clive A. ; DeMartino, George N. ; Hendil, Klavs B. ; Chung, Chin H. ; Tanahashi, Nobuyuki ; Tanaka, Keiji. / cDNA cloning and characterization of a human proteasomal modulator subunit, p27 (PSMD9). In: Genomics. 1998 ; Vol. 50, No. 2. pp. 241-250.
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