cDNA cloning and chromosomal localization of the human and mouse isoforms of Ksp-cadherin

R. B. Thomson, D. C. Ward, S. E. Quaggin, P. Igarashi, Z. E. Muckler, P. S. Aronson

Research output: Contribution to journalArticle

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Abstract

Ksp-cadherin is a novel kidney-specific member of the cadherin superfamily of cell adhesion molecules. We have determined the complete cDNA coding sequences of both the human and the mouse isoforms of Ksp-cadherin. The inferred amino acid sequences of the human and mouse isoforms are 79 and 75% identical to the originally described rabbit isoform of Ksp-cadherin (Thomson et al., 1995; J. Biol. Chem. 270, 17594-17601), respectively. The relative locations of cadherin-specific sequence motifs, putative N- glycosylation sites, and characteristic protein domains are entirely conserved in all three isoforms. Multiple organ Northern analyses indicate that, as in the rabbit, both the human and the mouse Ksp-cadherin transcripts appear to have distinct kidney-specific distributions. The human Ksp-cadherin gene (CDH16) maps to chromosome 16q21-proximal 16q22. The mouse Ksp-cadherin gene (Cdh16) was localized to a highly syntenic region of distal chromosome 8. Both the human and the mouse Ksp-cadherin genes were localized to previously identified clusters of cadherin gene sequences, consistent with the hypothesis that most cadherin family members arose by gene duplication from a single ancestral gene at a relatively early stage in the evolution of the mammalian genome.

Original languageEnglish (US)
Pages (from-to)445-451
Number of pages7
JournalGenomics
Volume51
Issue number3
DOIs
StatePublished - Aug 1 1998

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Cadherins
Organism Cloning
Protein Isoforms
Complementary DNA
Genes
Rabbits
Kidney
Chromosomes, Human, Pair 8
Gene Duplication
Cell Adhesion Molecules
Multigene Family
Glycosylation
Amino Acid Sequence
Chromosomes
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Thomson, R. B., Ward, D. C., Quaggin, S. E., Igarashi, P., Muckler, Z. E., & Aronson, P. S. (1998). cDNA cloning and chromosomal localization of the human and mouse isoforms of Ksp-cadherin. Genomics, 51(3), 445-451. https://doi.org/10.1006/geno.1998.5402

cDNA cloning and chromosomal localization of the human and mouse isoforms of Ksp-cadherin. / Thomson, R. B.; Ward, D. C.; Quaggin, S. E.; Igarashi, P.; Muckler, Z. E.; Aronson, P. S.

In: Genomics, Vol. 51, No. 3, 01.08.1998, p. 445-451.

Research output: Contribution to journalArticle

Thomson, RB, Ward, DC, Quaggin, SE, Igarashi, P, Muckler, ZE & Aronson, PS 1998, 'cDNA cloning and chromosomal localization of the human and mouse isoforms of Ksp-cadherin', Genomics, vol. 51, no. 3, pp. 445-451. https://doi.org/10.1006/geno.1998.5402
Thomson RB, Ward DC, Quaggin SE, Igarashi P, Muckler ZE, Aronson PS. cDNA cloning and chromosomal localization of the human and mouse isoforms of Ksp-cadherin. Genomics. 1998 Aug 1;51(3):445-451. https://doi.org/10.1006/geno.1998.5402
Thomson, R. B. ; Ward, D. C. ; Quaggin, S. E. ; Igarashi, P. ; Muckler, Z. E. ; Aronson, P. S. / cDNA cloning and chromosomal localization of the human and mouse isoforms of Ksp-cadherin. In: Genomics. 1998 ; Vol. 51, No. 3. pp. 445-451.
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