cDNA cloning and chromosomal localization of the human and mouse isoforms of Ksp-cadherin

R. B. Thomson, D. C. Ward, S. E. Quaggin, P. Igarashi, Z. E. Muckler, P. S. Aronson

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Abstract

Ksp-cadherin is a novel kidney-specific member of the cadherin superfamily of cell adhesion molecules. We have determined the complete cDNA coding sequences of both the human and the mouse isoforms of Ksp-cadherin. The inferred amino acid sequences of the human and mouse isoforms are 79 and 75% identical to the originally described rabbit isoform of Ksp-cadherin (Thomson et al., 1995; J. Biol. Chem. 270, 17594-17601), respectively. The relative locations of cadherin-specific sequence motifs, putative N- glycosylation sites, and characteristic protein domains are entirely conserved in all three isoforms. Multiple organ Northern analyses indicate that, as in the rabbit, both the human and the mouse Ksp-cadherin transcripts appear to have distinct kidney-specific distributions. The human Ksp-cadherin gene (CDH16) maps to chromosome 16q21-proximal 16q22. The mouse Ksp-cadherin gene (Cdh16) was localized to a highly syntenic region of distal chromosome 8. Both the human and the mouse Ksp-cadherin genes were localized to previously identified clusters of cadherin gene sequences, consistent with the hypothesis that most cadherin family members arose by gene duplication from a single ancestral gene at a relatively early stage in the evolution of the mammalian genome.

Original languageEnglish (US)
Pages (from-to)445-451
Number of pages7
JournalGenomics
Volume51
Issue number3
DOIs
StatePublished - Aug 1 1998

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ASJC Scopus subject areas

  • Genetics

Cite this

Thomson, R. B., Ward, D. C., Quaggin, S. E., Igarashi, P., Muckler, Z. E., & Aronson, P. S. (1998). cDNA cloning and chromosomal localization of the human and mouse isoforms of Ksp-cadherin. Genomics, 51(3), 445-451. https://doi.org/10.1006/geno.1998.5402