cDNA cloning and expression of the α and β subunits of rat rab geranylgeranyl transferase

Scott A. Armstrong, Miguel C. Seabra, Thomas C. Südhof, Joseph L. Goldstein, Michael S. Brown

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Rab geranylgeranyl transferasse (Rab GG transferase) attaches 20-carbon geranylgeranyl groups to cysteine residues in Rab proteins that contain the COOH-terminal sequence Cys-X-Cys or Cys-Cys. Rab GG transferase consists of two components that are separable in high salt solutions. Component A is a 95-kDa protein, and Component B is a heterodimer consisting of a 60-kDa α subunit and a 38-kDa β subunit. In the current paper, we have cloned cDNAs for the α and β subunits of Component B. The cDNAs for the rat α and β subunits predict proteins of 567 and 331 amino acids, respectively. The mRNAs for both subunits are expressed in many tissues. When transfected together in embryonic kidney 293 cells, the α and β subunit cDNAs produced Rab GG transferase activity that was stimulated in vitro by the addition of purified Component A. Comparisons of the amino acid sequences suggest that the proteins encoded by the Saccharomyces cerevisiae genes MAD2 and BET2 are the yeast counterparts of the mammalian Rab GG transferase α and β subunits, respectively.

Original languageEnglish (US)
Pages (from-to)12221-12229
Number of pages9
JournalJournal of Biological Chemistry
Volume268
Issue number16
StatePublished - Jun 5 1993

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Cloning
Rats
Organism Cloning
Complementary DNA
Yeast
cysteinylcysteine
Saccharomyces cerevisiae Proteins
Amino Acids
Proteins
Protein Subunits
Cysteine
Amino Acid Sequence
Carbon
Salts
Genes
Yeasts
Tissue
Kidney
Messenger RNA
Rab geranylgeranyltransferase

ASJC Scopus subject areas

  • Biochemistry

Cite this

cDNA cloning and expression of the α and β subunits of rat rab geranylgeranyl transferase. / Armstrong, Scott A.; Seabra, Miguel C.; Südhof, Thomas C.; Goldstein, Joseph L.; Brown, Michael S.

In: Journal of Biological Chemistry, Vol. 268, No. 16, 05.06.1993, p. 12221-12229.

Research output: Contribution to journalArticle

Armstrong, Scott A. ; Seabra, Miguel C. ; Südhof, Thomas C. ; Goldstein, Joseph L. ; Brown, Michael S. / cDNA cloning and expression of the α and β subunits of rat rab geranylgeranyl transferase. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 16. pp. 12221-12229.
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AB - Rab geranylgeranyl transferasse (Rab GG transferase) attaches 20-carbon geranylgeranyl groups to cysteine residues in Rab proteins that contain the COOH-terminal sequence Cys-X-Cys or Cys-Cys. Rab GG transferase consists of two components that are separable in high salt solutions. Component A is a 95-kDa protein, and Component B is a heterodimer consisting of a 60-kDa α subunit and a 38-kDa β subunit. In the current paper, we have cloned cDNAs for the α and β subunits of Component B. The cDNAs for the rat α and β subunits predict proteins of 567 and 331 amino acids, respectively. The mRNAs for both subunits are expressed in many tissues. When transfected together in embryonic kidney 293 cells, the α and β subunit cDNAs produced Rab GG transferase activity that was stimulated in vitro by the addition of purified Component A. Comparisons of the amino acid sequences suggest that the proteins encoded by the Saccharomyces cerevisiae genes MAD2 and BET2 are the yeast counterparts of the mammalian Rab GG transferase α and β subunits, respectively.

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