cDNA cloning of a myosin heavy chain isoform in embryonic smooth muscle and its expression during vascular development and in arteriosclerosis

Makoto Kuro-o, Ryozo Nagai, Ken Ichi Nakahara, Hirohisa Katoh, Rong Chi Tsai, Hidetsugu Tsuchimochi, Yoshio Yazaki, Akiyuki Ohkubo, Fumimaro Takaku

Research output: Contribution to journalArticle

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Abstract

Adult rabbit smooth muscles contain two types of myosin heavy chain (MHC) isoforms, SM1 and SM2, which are generated through alternative RNA splicing from a single gene (Nagai, R., Kuro-o, M., Babij, P. & Periasamy, M. (1989) J. Biol. Chem. 264, 9734-9737). We previously reported that the expression of SM1 and SM2 during vascular development is differentially regulated at the level of RNA splicing, whereby SM1 is constitutively expressed from early development but SM2 appear after birth (Kuro-o, M., Nagai, R., Tsuchimochi, H., Katoh, H., Yazaki, Y., Ohkubo, A. & Takaku, F. (1989) J. Biol. Chem. 264, 18272-18275). We also demonstrated that embryonic vascular smooth muscles contain a third type of MHC isoform, referred to as SMemb in this report, which comigrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with SM2. In the present study we have isolated and characterized a cDNA clone (FSMHC34) for SMemb. FSMHC34 encodes the light meromyosin region including the carboxyl terminus and showed 70% amino acid sequence identity with SM1 or SM2. SMemb is a nonmuscle-type MHC and identical with brain MHC, but clearly distinct from 196-kDa nonmuscle MHC in cultured smooth muscle cells. The expression of SMemb was predominant in embryonic and perinatal aortas, but down-regulated with vascular development. Interestingly SMemb was reexpressed in proliferating smooth muscle cells of arteriosclerotic neointimas. These results suggest that smooth muscle proliferation is coupled to the expression of SMemb and that dedifferentiation of smooth muscles toward the embryonic phenotype is involved in the mechanisms underlying atherosclerosis.

Original languageEnglish (US)
Pages (from-to)3768-3773
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number6
StatePublished - Feb 25 1991

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Myosin Heavy Chains
Arteriosclerosis
Cloning
Smooth Muscle
Blood Vessels
Muscle
Organism Cloning
Protein Isoforms
Complementary DNA
Smooth Muscle Myocytes
vpr Genes
RNA Splicing
Myosin Subfragments
Cells
Alternative Splicing
Vascular Smooth Muscle
Sodium Dodecyl Sulfate
Aorta
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence

ASJC Scopus subject areas

  • Biochemistry

Cite this

cDNA cloning of a myosin heavy chain isoform in embryonic smooth muscle and its expression during vascular development and in arteriosclerosis. / Kuro-o, Makoto; Nagai, Ryozo; Nakahara, Ken Ichi; Katoh, Hirohisa; Tsai, Rong Chi; Tsuchimochi, Hidetsugu; Yazaki, Yoshio; Ohkubo, Akiyuki; Takaku, Fumimaro.

In: Journal of Biological Chemistry, Vol. 266, No. 6, 25.02.1991, p. 3768-3773.

Research output: Contribution to journalArticle

Kuro-o, M, Nagai, R, Nakahara, KI, Katoh, H, Tsai, RC, Tsuchimochi, H, Yazaki, Y, Ohkubo, A & Takaku, F 1991, 'cDNA cloning of a myosin heavy chain isoform in embryonic smooth muscle and its expression during vascular development and in arteriosclerosis', Journal of Biological Chemistry, vol. 266, no. 6, pp. 3768-3773.
Kuro-o, Makoto ; Nagai, Ryozo ; Nakahara, Ken Ichi ; Katoh, Hirohisa ; Tsai, Rong Chi ; Tsuchimochi, Hidetsugu ; Yazaki, Yoshio ; Ohkubo, Akiyuki ; Takaku, Fumimaro. / cDNA cloning of a myosin heavy chain isoform in embryonic smooth muscle and its expression during vascular development and in arteriosclerosis. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 6. pp. 3768-3773.
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AU - Tsai, Rong Chi

AU - Tsuchimochi, Hidetsugu

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AU - Takaku, Fumimaro

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