cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA

Takeshi Sakurai, Masashi Yanagisawa, Akihiro Inoue, Una S. Ryan, Sadao Kimura, Youji Mitsui, Katsutoshi Goto, Tomoh Masaki

Research output: Contribution to journalArticlepeer-review

234 Scopus citations


We report the cloning of a full-length cDNA encoding rat preproendothelin-1 (preproET-1). The predicted rat preproET-1 consists of 202 amino acid residues and highly similar to human, porcine and bovine preproET-1, respectively. The deduced 21-residue sequence of mature rat ET-1 is identical to human, porcine, canine and bovine ET-1. As in other mammalian species, the mature ET-1 is predicted to be produced from a 39-residue big ET-1 in the rat. Northern blot analysis showed that a single 2.3-kb preproET-1 mRNA is expressed not only in vascular endothelial cells but also in other rat tissues, including the lung, brain, uterus, stomach, heart, adrenal gland and kidney. These findings suggest that ET-1 may play roles as a local mediator in multiple organs both within and outside the cardiovascular system in the rat.

Original languageEnglish (US)
Pages (from-to)44-47
Number of pages4
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Feb 28 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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