C/EBP-beta drives expression of the nutritionally regulated promoter IA of the acetyl-CoA carboxylase-alpha gene in cattle

Xuanming Shi, Shuzhen Liu, Cornelia C. Metges, Hans Martin Seyfert

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Acetyl-CoA carboxylase-alpha (ACC-α) is the rate-limiting enzyme for de novo fatty acid synthesis. Among the four promoters expressing the bovine gene, promoter IA (PIA) is dominantly active and nutritionally regulated in lipogenic tissues. CCAAT/enhancer binding proteins are crucially involved in regulating the activity of this promoter. We examine here, which member of this family of transcription factors is most important for promoter activation. To differentiate the individual contribution of different members of the C/EBP family transcription factors controlling the ACC-α gene expression in cattle, we established vectors expressing full length (FL) or N-terminally truncated (δN) variants of the C/EBP factors (α, -β, -γ, and -ε) in mammalian cells. Using nuclear extracts of cells expressing the δN-C/EBP factors we determined in electrophoretic mobility shift assays that C/EBPα, -β and -ε, but not C/EBPγ may directly bind to the cognate C/EBP-binding site in the immediate ACC-α PIA. Co-transfection analyses of the various FL-C/EBP expression vectors together with a reporter gene driven by the ACC-α-PIA promoter demonstrated that C/EBPβ has the strongest activation potential. Hence, activity of this factor may be a key regulator of ACC-α-expression in lipogenic tissues.

Original languageEnglish (US)
Pages (from-to)561-567
Number of pages7
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1799
Issue number8
DOIs
StatePublished - Aug 2010

Keywords

  • ACC-alpha
  • C/EBP
  • Cattle
  • Gene expression
  • Isoform
  • Lipid synthesis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics

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