Cell-Based Screen Identifies Human Interferon-Stimulated Regulators of Listeria monocytogenes Infection

Sofya S. Perelman, Michael E. Abrams, Jennifer L. Eitson, Didi Chen, Alyssa Jimenez, Marcel Mettlen, John W. Schoggins, Neal M. Alto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The type I interferon (IFN) activated transcriptional response is a critical antiviral defense mechanism, yet its role in bacterial pathogenesis remains less well characterized. Using an intracellular pathogen Listeria monocytogenes (Lm) as a model bacterial pathogen, we sought to identify the roles of individual interferon-stimulated genes (ISGs) in context of bacterial infection. Previously, IFN has been implicated in both restricting and promoting Lm growth and immune stimulatory functions in vivo. Here we adapted a gain-of-function flow cytometry based approach to screen a library of more than 350 human ISGs for inhibitors and enhancers of Lm infection. We identify 6 genes, including UNC93B1, MYD88, AQP9, and TRIM14 that potently inhibit Lm infection. These inhibitors act through both transcription-mediated (MYD88) and non-transcriptional mechanisms (TRIM14). Further, we identify and characterize the human high affinity immunoglobulin receptor FcγRIa as an enhancer of Lm internalization. Our results reveal that FcγRIa promotes Lm uptake in the absence of known host Lm internalization receptors (E-cadherin and c-Met) as well as bacterial surface internalins (InlA and InlB). Additionally, FcγRIa-mediated uptake occurs independently of Lm opsonization or canonical FcγRIa signaling. Finally, we established the contribution of FcγRIa to Lm infection in phagocytic cells, thus potentially linking the IFN response to a novel bacterial uptake pathway. Together, these studies provide an experimental and conceptual basis for deciphering the role of IFN in bacterial defense and virulence at single-gene resolution.

Original languageEnglish (US)
Article numbere1006102
JournalPLoS Pathogens
Volume12
Issue number12
DOIs
StatePublished - Dec 21 2016

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Listeriosis
Listeria monocytogenes
Interferons
Genes
Interferon Type I
Cadherins
Phagocytes
Bacterial Infections
Libraries
Antiviral Agents
Virulence
Immunoglobulins
Flow Cytometry

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Cell-Based Screen Identifies Human Interferon-Stimulated Regulators of Listeria monocytogenes Infection. / Perelman, Sofya S.; Abrams, Michael E.; Eitson, Jennifer L.; Chen, Didi; Jimenez, Alyssa; Mettlen, Marcel; Schoggins, John W.; Alto, Neal M.

In: PLoS Pathogens, Vol. 12, No. 12, e1006102, 21.12.2016.

Research output: Contribution to journalArticle

Perelman, Sofya S. ; Abrams, Michael E. ; Eitson, Jennifer L. ; Chen, Didi ; Jimenez, Alyssa ; Mettlen, Marcel ; Schoggins, John W. ; Alto, Neal M. / Cell-Based Screen Identifies Human Interferon-Stimulated Regulators of Listeria monocytogenes Infection. In: PLoS Pathogens. 2016 ; Vol. 12, No. 12.
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