Cell culture methods for the establishment of the NCI series of lung cancer cell lines

Herbert K. Oie, Edward K. Russell, Desmond N. Carney, Adi F. Gazdar

Research output: Contribution to journalArticle

Abstract

More than 200 human small cell lung cancer and non-small cell lung cancer cell lines were established over 15 years mainly by utilizing the serum-free, hormone and growth factor supplemented, defined media HITES and ACL4. Use of modified, established cell culture techniques such as the mechanical spillout method for the releasing of cell aggregates from tumor tissue, ficoll gradient centrifugation for the separation of tumor cells from erythrocytes and tissue debris, and an apparatue consisting of a platinum tubing attached to a suction flask for removal of spent medium have greatly contributed to the success in culturing tumor cells. Characterization of these lung cancer cell lines have extended our knowledge of lung cell biology. Studies elucidating the nutritional requirements of lung cancer cell growth may be helpful for the manipulation of these tumors in patients.

Original languageEnglish (US)
Pages (from-to)24-31
Number of pages8
JournalJournal of Cellular Biochemistry
Volume62
Issue numberSUPPL. 24
StatePublished - 1996

Fingerprint

Cell culture
Lung Neoplasms
Cell Culture Techniques
Cells
Cell Line
Tumors
Neoplasms
Nutritional Requirements
Ficoll
Cell Separation
Small Cell Lung Carcinoma
Suction
Platinum
Centrifugation
Non-Small Cell Lung Carcinoma
Cytology
Tissue
Cell Biology
Intercellular Signaling Peptides and Proteins
Erythrocytes

Keywords

  • cell culture techniques
  • defined medium
  • lung cancer cell lines
  • SErum-free

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Oie, H. K., Russell, E. K., Carney, D. N., & Gazdar, A. F. (1996). Cell culture methods for the establishment of the NCI series of lung cancer cell lines. Journal of Cellular Biochemistry, 62(SUPPL. 24), 24-31.

Cell culture methods for the establishment of the NCI series of lung cancer cell lines. / Oie, Herbert K.; Russell, Edward K.; Carney, Desmond N.; Gazdar, Adi F.

In: Journal of Cellular Biochemistry, Vol. 62, No. SUPPL. 24, 1996, p. 24-31.

Research output: Contribution to journalArticle

Oie, HK, Russell, EK, Carney, DN & Gazdar, AF 1996, 'Cell culture methods for the establishment of the NCI series of lung cancer cell lines', Journal of Cellular Biochemistry, vol. 62, no. SUPPL. 24, pp. 24-31.
Oie, Herbert K. ; Russell, Edward K. ; Carney, Desmond N. ; Gazdar, Adi F. / Cell culture methods for the establishment of the NCI series of lung cancer cell lines. In: Journal of Cellular Biochemistry. 1996 ; Vol. 62, No. SUPPL. 24. pp. 24-31.
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