Cell cycle-dependent protein expression of mammalian homologs of yeast DNA double-strand break repair genes Rad51 and Rad52

Fanqing Chen, Anthony Nastasi, Zhiyuan Shen, Mark Brenneman, Harry Crissman, David J. Chen

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

Recently, human and rodent homologs of yeast repair genes Rad51 and Rad52 have been identified and proposed to play roles in DNA double-strand break (DSB) repair. In this study, cell cycle-dependent expression of human and rodent RAD51 and RAD52 proteins was monitored using two approaches. First, flow cytometric measurements of DNA content and immunofluorescence were used to determine the phase-specific levels of RAD51 and RAD52 protein expression in irradiated and control populations. The expression of both proteins was lowest in G0/G1, increased in S and reached a maximum in G2/M. No difference was found in the whole-cell level of RAD51 or RAD52 protein expression between γ-irradiated and control cell populations. Second, cell cycle-dependent protein expression was confirmed by Western analysis of populations synchronized in G0, G1 and G2 phases. Analysis of V3, a hamster equivalent of SCID, indicates that the protein level increases of RAD51 and RAD52 from G0 to G1/S/G2 do not require DNA-PK.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalMutation Research - DNA Repair
Volume384
Issue number3
DOIs
StatePublished - Sep 1 1997

Keywords

  • Cell cycle
  • DNA double strand break repair
  • Rad51
  • Rad52
  • Radiation

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

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