Cell cycle-dependent protein expression of mammalian homologs of yeast DNA double-strand break repair genes Rad51 and Rad52

Fanqing Chen, Anthony Nastasi, Zhiyuan Shen, Mark Brenneman, Harry Crissman, David J. Chen

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Recently, human and rodent homologs of yeast repair genes Rad51 and Rad52 have been identified and proposed to play roles in DNA double-strand break (DSB) repair. In this study, cell cycle-dependent expression of human and rodent RAD51 and RAD52 proteins was monitored using two approaches. First, flow cytometric measurements of DNA content and immunofluorescence were used to determine the phase-specific levels of RAD51 and RAD52 protein expression in irradiated and control populations. The expression of both proteins was lowest in G0/G1, increased in S and reached a maximum in G2/M. No difference was found in the whole-cell level of RAD51 or RAD52 protein expression between γ-irradiated and control cell populations. Second, cell cycle-dependent protein expression was confirmed by Western analysis of populations synchronized in G0, G1 and G2 phases. Analysis of V3, a hamster equivalent of SCID, indicates that the protein level increases of RAD51 and RAD52 from G0 to G1/S/G2 do not require DNA-PK.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalMutation Research - DNA Repair
Volume384
Issue number3
DOIs
StatePublished - Sep 1997

Fingerprint

Cell Cycle Proteins
Double-Stranded DNA Breaks
Yeast
Repair
Genes
Yeasts
Cells
Rodentia
DNA
Rad51 Recombinase
Population
Cell Cycle Resting Phase
Proteins
G2 Phase
G1 Phase
Cricetinae
Fluorescent Antibody Technique
Cell Cycle
Flow measurement

Keywords

  • Cell cycle
  • DNA double strand break repair
  • Rad51
  • Rad52
  • Radiation

ASJC Scopus subject areas

  • Toxicology
  • Genetics
  • Molecular Biology

Cite this

Cell cycle-dependent protein expression of mammalian homologs of yeast DNA double-strand break repair genes Rad51 and Rad52. / Chen, Fanqing; Nastasi, Anthony; Shen, Zhiyuan; Brenneman, Mark; Crissman, Harry; Chen, David J.

In: Mutation Research - DNA Repair, Vol. 384, No. 3, 09.1997, p. 205-211.

Research output: Contribution to journalArticle

Chen, Fanqing ; Nastasi, Anthony ; Shen, Zhiyuan ; Brenneman, Mark ; Crissman, Harry ; Chen, David J. / Cell cycle-dependent protein expression of mammalian homologs of yeast DNA double-strand break repair genes Rad51 and Rad52. In: Mutation Research - DNA Repair. 1997 ; Vol. 384, No. 3. pp. 205-211.
@article{187952ee652646d783e3d294aac58420,
title = "Cell cycle-dependent protein expression of mammalian homologs of yeast DNA double-strand break repair genes Rad51 and Rad52",
abstract = "Recently, human and rodent homologs of yeast repair genes Rad51 and Rad52 have been identified and proposed to play roles in DNA double-strand break (DSB) repair. In this study, cell cycle-dependent expression of human and rodent RAD51 and RAD52 proteins was monitored using two approaches. First, flow cytometric measurements of DNA content and immunofluorescence were used to determine the phase-specific levels of RAD51 and RAD52 protein expression in irradiated and control populations. The expression of both proteins was lowest in G0/G1, increased in S and reached a maximum in G2/M. No difference was found in the whole-cell level of RAD51 or RAD52 protein expression between γ-irradiated and control cell populations. Second, cell cycle-dependent protein expression was confirmed by Western analysis of populations synchronized in G0, G1 and G2 phases. Analysis of V3, a hamster equivalent of SCID, indicates that the protein level increases of RAD51 and RAD52 from G0 to G1/S/G2 do not require DNA-PK.",
keywords = "Cell cycle, DNA double strand break repair, Rad51, Rad52, Radiation",
author = "Fanqing Chen and Anthony Nastasi and Zhiyuan Shen and Mark Brenneman and Harry Crissman and Chen, {David J.}",
year = "1997",
month = "9",
doi = "10.1016/S0921-8777(97)00020-7",
language = "English (US)",
volume = "384",
pages = "205--211",
journal = "Mutation Research - DNA Repair",
issn = "0921-8777",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - Cell cycle-dependent protein expression of mammalian homologs of yeast DNA double-strand break repair genes Rad51 and Rad52

AU - Chen, Fanqing

AU - Nastasi, Anthony

AU - Shen, Zhiyuan

AU - Brenneman, Mark

AU - Crissman, Harry

AU - Chen, David J.

PY - 1997/9

Y1 - 1997/9

N2 - Recently, human and rodent homologs of yeast repair genes Rad51 and Rad52 have been identified and proposed to play roles in DNA double-strand break (DSB) repair. In this study, cell cycle-dependent expression of human and rodent RAD51 and RAD52 proteins was monitored using two approaches. First, flow cytometric measurements of DNA content and immunofluorescence were used to determine the phase-specific levels of RAD51 and RAD52 protein expression in irradiated and control populations. The expression of both proteins was lowest in G0/G1, increased in S and reached a maximum in G2/M. No difference was found in the whole-cell level of RAD51 or RAD52 protein expression between γ-irradiated and control cell populations. Second, cell cycle-dependent protein expression was confirmed by Western analysis of populations synchronized in G0, G1 and G2 phases. Analysis of V3, a hamster equivalent of SCID, indicates that the protein level increases of RAD51 and RAD52 from G0 to G1/S/G2 do not require DNA-PK.

AB - Recently, human and rodent homologs of yeast repair genes Rad51 and Rad52 have been identified and proposed to play roles in DNA double-strand break (DSB) repair. In this study, cell cycle-dependent expression of human and rodent RAD51 and RAD52 proteins was monitored using two approaches. First, flow cytometric measurements of DNA content and immunofluorescence were used to determine the phase-specific levels of RAD51 and RAD52 protein expression in irradiated and control populations. The expression of both proteins was lowest in G0/G1, increased in S and reached a maximum in G2/M. No difference was found in the whole-cell level of RAD51 or RAD52 protein expression between γ-irradiated and control cell populations. Second, cell cycle-dependent protein expression was confirmed by Western analysis of populations synchronized in G0, G1 and G2 phases. Analysis of V3, a hamster equivalent of SCID, indicates that the protein level increases of RAD51 and RAD52 from G0 to G1/S/G2 do not require DNA-PK.

KW - Cell cycle

KW - DNA double strand break repair

KW - Rad51

KW - Rad52

KW - Radiation

UR - http://www.scopus.com/inward/record.url?scp=0030801803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030801803&partnerID=8YFLogxK

U2 - 10.1016/S0921-8777(97)00020-7

DO - 10.1016/S0921-8777(97)00020-7

M3 - Article

C2 - 9330616

AN - SCOPUS:0030801803

VL - 384

SP - 205

EP - 211

JO - Mutation Research - DNA Repair

JF - Mutation Research - DNA Repair

SN - 0921-8777

IS - 3

ER -