Cloned, neoplastic B cells (BCL1) have been used to evaluate the expression of the receptor for the B cell differentiation factor, BCDFμ. These cells do not secrete IgM before stimulation with BCDFμ-containing T cell supernatants (SN). By inducing cell cycle synchrony in this homogeneous population, the expression of the BCDFμ receptor could be evaluated as a function of the cell cycle. Responsiveness to BCDFμ-containing SN is maximal when the cells are in S and G2 phases of the cell cycle, and a 2-hr exposure of cells to BCDFμ-containing SN during S/G2 results in optimal IgM secretion 5 days later. Cells in S/G2 are also maximally effective in absorbing BCDFμ activity from SN. These data support the hypothesis that B cells do not respond to differentiative signals until after they are committed to at least one round of cell division.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1985|
ASJC Scopus subject areas
- Immunology and Allergy