Cell encapsulation and oxygenation in nanoporous microcontainers

Barjor Gimi, Joonbum Kwon, Li Liu, Yang Su, Krishnamurthy Nemani, Krutarth Trivedi, Yonghao Cui, Behroze Vachha, Ralph Mason, Wenchuang Hu, Jeong Bong Lee

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

With strides in stem cell biology, cell engineering and molecular therapy, the transplantation of cells to produce therapeutic molecules endogenously is an attractive and achievable alternative to the use of exogenous drugs. The encapsulation of such cell transplants in semi-permeable, nanoporous constructs is often required to protect them from immune attack and to prevent their proliferation in the host. However, effective graft immunoisolation has been mostly elusive owing to the absence of a high-throughput method to create precisely controlled, high-aspect-ratio nanopores. To address the clinical need for effective cell encapsulation and immunoisolation, we devised a biocompatible cell-encapsulating microcontainer and a method to create highly anisotropic nanopores in the microcontainer's surface. To evaluate the efficacy of these nanopores in oxygenating the encapsulated cells, we engineered 9L rat glioma cells to bioluminesce under hypoxic conditions. The methods described above should aid in evaluating the long term survival and efficacy of cellular grafts.

Original languageEnglish (US)
Pages (from-to)1205-1212
Number of pages8
JournalBiomedical Microdevices
Volume11
Issue number6
DOIs
StatePublished - Dec 1 2009

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Keywords

  • Cell encapsulation therapy
  • Cell therapy
  • Immunoisolation
  • Immunoprotection
  • Microencapsulation
  • Nanoporosity

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

Cite this

Gimi, B., Kwon, J., Liu, L., Su, Y., Nemani, K., Trivedi, K., Cui, Y., Vachha, B., Mason, R., Hu, W., & Lee, J. B. (2009). Cell encapsulation and oxygenation in nanoporous microcontainers. Biomedical Microdevices, 11(6), 1205-1212. https://doi.org/10.1007/s10544-009-9338-0