Cell-intrinsic differences between stem cells from different regions of the peripheral nervous system regulate the generation of neural diversity

Suzanne Bixby, Genevieve M. Kruger, Jack T. Mosher, Nancy M. Joseph, Sean J. Morrison

Research output: Contribution to journalArticle

229 Citations (Scopus)

Abstract

Stem cells in different regions of the nervous system give rise to different types of mature cells. This diversity is assumed to arise in response to local environmental differences, but the contribution of cell-intrinsic differences between stem cells has been unclear. At embryonic day (E)14, neural crest stem cells (NCSCs) undergo primarily neurogenesis in the gut but gliogenesis in nerves. Yet gliogenic and neurogenic factors are expressed in both locations. NCSCs isolated by flow-cytometry from E14 sciatic nerve and gut exhibited heritable, cell-intrinsic differences in their responsiveness to lineage determination factors. Gut NCSCs were more responsive to neurogenic factors, while sciatic nerve NCSCs were more responsive to gliogenic factors. Upon transplantation of uncultured NCSCs into developing peripheral nerves in vivo, sciatic nerve NCSCs gave rise only to glia, while gut NCSCs gave rise primarily to neurons. Thus, cell fate in the nerve was stem cell determined.

Original languageEnglish (US)
Pages (from-to)643-656
Number of pages14
JournalNeuron
Volume35
Issue number4
DOIs
StatePublished - Aug 15 2002

Fingerprint

Neural Stem Cells
Neural Crest
Peripheral Nervous System
Stem Cells
Sciatic Nerve
Neurogenesis
Peripheral Nerves
Neuroglia
Nervous System
Flow Cytometry
Transplantation
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cell-intrinsic differences between stem cells from different regions of the peripheral nervous system regulate the generation of neural diversity. / Bixby, Suzanne; Kruger, Genevieve M.; Mosher, Jack T.; Joseph, Nancy M.; Morrison, Sean J.

In: Neuron, Vol. 35, No. 4, 15.08.2002, p. 643-656.

Research output: Contribution to journalArticle

Bixby, Suzanne ; Kruger, Genevieve M. ; Mosher, Jack T. ; Joseph, Nancy M. ; Morrison, Sean J. / Cell-intrinsic differences between stem cells from different regions of the peripheral nervous system regulate the generation of neural diversity. In: Neuron. 2002 ; Vol. 35, No. 4. pp. 643-656.
@article{1e8ad76c2e0a488c98117e848a438e17,
title = "Cell-intrinsic differences between stem cells from different regions of the peripheral nervous system regulate the generation of neural diversity",
abstract = "Stem cells in different regions of the nervous system give rise to different types of mature cells. This diversity is assumed to arise in response to local environmental differences, but the contribution of cell-intrinsic differences between stem cells has been unclear. At embryonic day (E)14, neural crest stem cells (NCSCs) undergo primarily neurogenesis in the gut but gliogenesis in nerves. Yet gliogenic and neurogenic factors are expressed in both locations. NCSCs isolated by flow-cytometry from E14 sciatic nerve and gut exhibited heritable, cell-intrinsic differences in their responsiveness to lineage determination factors. Gut NCSCs were more responsive to neurogenic factors, while sciatic nerve NCSCs were more responsive to gliogenic factors. Upon transplantation of uncultured NCSCs into developing peripheral nerves in vivo, sciatic nerve NCSCs gave rise only to glia, while gut NCSCs gave rise primarily to neurons. Thus, cell fate in the nerve was stem cell determined.",
author = "Suzanne Bixby and Kruger, {Genevieve M.} and Mosher, {Jack T.} and Joseph, {Nancy M.} and Morrison, {Sean J.}",
year = "2002",
month = "8",
day = "15",
doi = "10.1016/S0896-6273(02)00825-5",
language = "English (US)",
volume = "35",
pages = "643--656",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Cell-intrinsic differences between stem cells from different regions of the peripheral nervous system regulate the generation of neural diversity

AU - Bixby, Suzanne

AU - Kruger, Genevieve M.

AU - Mosher, Jack T.

AU - Joseph, Nancy M.

AU - Morrison, Sean J.

PY - 2002/8/15

Y1 - 2002/8/15

N2 - Stem cells in different regions of the nervous system give rise to different types of mature cells. This diversity is assumed to arise in response to local environmental differences, but the contribution of cell-intrinsic differences between stem cells has been unclear. At embryonic day (E)14, neural crest stem cells (NCSCs) undergo primarily neurogenesis in the gut but gliogenesis in nerves. Yet gliogenic and neurogenic factors are expressed in both locations. NCSCs isolated by flow-cytometry from E14 sciatic nerve and gut exhibited heritable, cell-intrinsic differences in their responsiveness to lineage determination factors. Gut NCSCs were more responsive to neurogenic factors, while sciatic nerve NCSCs were more responsive to gliogenic factors. Upon transplantation of uncultured NCSCs into developing peripheral nerves in vivo, sciatic nerve NCSCs gave rise only to glia, while gut NCSCs gave rise primarily to neurons. Thus, cell fate in the nerve was stem cell determined.

AB - Stem cells in different regions of the nervous system give rise to different types of mature cells. This diversity is assumed to arise in response to local environmental differences, but the contribution of cell-intrinsic differences between stem cells has been unclear. At embryonic day (E)14, neural crest stem cells (NCSCs) undergo primarily neurogenesis in the gut but gliogenesis in nerves. Yet gliogenic and neurogenic factors are expressed in both locations. NCSCs isolated by flow-cytometry from E14 sciatic nerve and gut exhibited heritable, cell-intrinsic differences in their responsiveness to lineage determination factors. Gut NCSCs were more responsive to neurogenic factors, while sciatic nerve NCSCs were more responsive to gliogenic factors. Upon transplantation of uncultured NCSCs into developing peripheral nerves in vivo, sciatic nerve NCSCs gave rise only to glia, while gut NCSCs gave rise primarily to neurons. Thus, cell fate in the nerve was stem cell determined.

UR - http://www.scopus.com/inward/record.url?scp=0037104626&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037104626&partnerID=8YFLogxK

U2 - 10.1016/S0896-6273(02)00825-5

DO - 10.1016/S0896-6273(02)00825-5

M3 - Article

C2 - 12194865

AN - SCOPUS:0037104626

VL - 35

SP - 643

EP - 656

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 4

ER -