Cell-matrix entanglement and mechanical anchorage of fibroblasts in three-dimensional collagen matrices

Hongmei Jiang, Frederick Grinnell

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Fibroblast-3D collagen matrix culture provides a physiologically relevant model to study cell-matrix interactions. In tissues, fibroblasts are phagocytic cells, and in culture, they have been shown to ingest both fibronectin and collagen-coated latex particles. Compared with cells on collagen-coated coverslips, phagocytosis of fibronectin-coated beads by fibroblasts in collagen matrices was found to be reduced. This decrease could not be explained by integrin reorganization, tight binding of fibronectin beads to the collagen matrix, or differences in overall bead binding to the cells. Rather, entanglement of cellular dendritic extensions with collagen fibrils seemed to interfere with the ability of the extensions to interact with the beads. Moreover, once these extensions became entangled in the matrix, cells developed an integrin-independent component of adhesion. We suggest that cell-matrix entanglement represents a novel mechanism of cell anchorage that uniquely depends on the three-dimensional character of the matrix.

Original languageEnglish (US)
Pages (from-to)5070-5076
Number of pages7
JournalMolecular Biology of the Cell
Volume16
Issue number11
DOIs
StatePublished - Nov 2005

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Collagen
Fibroblasts
Fibronectins
Integrins
Phagocytes
Microspheres
Phagocytosis
Cell Communication
Cell Culture Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cell-matrix entanglement and mechanical anchorage of fibroblasts in three-dimensional collagen matrices. / Jiang, Hongmei; Grinnell, Frederick.

In: Molecular Biology of the Cell, Vol. 16, No. 11, 11.2005, p. 5070-5076.

Research output: Contribution to journalArticle

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