TY - JOUR
T1 - Cell-matrix entanglement and mechanical anchorage of fibroblasts in three-dimensional collagen matrices
AU - Jiang, Hongmei
AU - Grinnell, Frederick
PY - 2005/11
Y1 - 2005/11
N2 - Fibroblast-3D collagen matrix culture provides a physiologically relevant model to study cell-matrix interactions. In tissues, fibroblasts are phagocytic cells, and in culture, they have been shown to ingest both fibronectin and collagen-coated latex particles. Compared with cells on collagen-coated coverslips, phagocytosis of fibronectin-coated beads by fibroblasts in collagen matrices was found to be reduced. This decrease could not be explained by integrin reorganization, tight binding of fibronectin beads to the collagen matrix, or differences in overall bead binding to the cells. Rather, entanglement of cellular dendritic extensions with collagen fibrils seemed to interfere with the ability of the extensions to interact with the beads. Moreover, once these extensions became entangled in the matrix, cells developed an integrin-independent component of adhesion. We suggest that cell-matrix entanglement represents a novel mechanism of cell anchorage that uniquely depends on the three-dimensional character of the matrix.
AB - Fibroblast-3D collagen matrix culture provides a physiologically relevant model to study cell-matrix interactions. In tissues, fibroblasts are phagocytic cells, and in culture, they have been shown to ingest both fibronectin and collagen-coated latex particles. Compared with cells on collagen-coated coverslips, phagocytosis of fibronectin-coated beads by fibroblasts in collagen matrices was found to be reduced. This decrease could not be explained by integrin reorganization, tight binding of fibronectin beads to the collagen matrix, or differences in overall bead binding to the cells. Rather, entanglement of cellular dendritic extensions with collagen fibrils seemed to interfere with the ability of the extensions to interact with the beads. Moreover, once these extensions became entangled in the matrix, cells developed an integrin-independent component of adhesion. We suggest that cell-matrix entanglement represents a novel mechanism of cell anchorage that uniquely depends on the three-dimensional character of the matrix.
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U2 - 10.1091/mbc.E05-01-0007
DO - 10.1091/mbc.E05-01-0007
M3 - Article
C2 - 16107563
AN - SCOPUS:27644570749
SN - 1059-1524
VL - 16
SP - 5070
EP - 5076
JO - Molecular biology of the cell
JF - Molecular biology of the cell
IS - 11
ER -